Effect of Glutamine Synthetase Gene Overexpression in Birch (Betula pubescens) Plants on Auxin Content and Rooting in vitro
| Autor: | Guzel R. Kudoyarova, A V Korobova, G V Shendel, V G Lebedev, K A Shestibratov |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biophysics Genetically modified crops 01 natural sciences Biochemistry Plant Roots Gene Expression Regulation Enzymologic 03 medical and health sciences Auxin Gene Expression Regulation Plant Glutamate-Ammonia Ligase Glutamine synthetase Botany Gene Betula Plant Proteins chemistry.chemical_classification Regulation of gene expression biology Indoleacetic Acids fungi food and beverages General Chemistry General Medicine Betula pubescens biology.organism_classification Plants Genetically Modified Glutamine Transformation (genetics) 030104 developmental biology chemistry 010606 plant biology & botany |
| Zdroj: | Doklady. Biochemistry and biophysics. 480(1) |
| ISSN: | 1608-3091 |
| Popis: | The effects of transformation of downy birch (Betula pubescens Ehrh.) with the GS1 gene encoding the cytosolic form of glutamine synthetase on the rooting of plants in vitro was studied. The transgenic plants had an elevated content of glutamine as well as glutamic and aspartic acids and rooted more rapidly than the control plants. Rooting on a medium containing the glutamine synthetase inhibitor phosphinothricin prevented the accumulation of auxin in birch plants carrying the GS1 gene, indicating the involvement of this enzyme in raising the level of auxins in the transgenic plants. The correlation between the increase in the auxin levels in the transgenic plants carrying the glutamine synthetase gene and the increase in the rooting rate is shown for the first time. |
| Databáze: | OpenAIRE |
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