Identification of a normal human bone marrow cell population co-exmessin-g megakaryocytic and erythroid markers in culture
| Autor: | Sylvia Bellucci, Dominique Pidard, Z. C. Han, J.P. Caen |
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| Rok vydání: | 2009 |
| Předmět: |
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Fluorescent Antibody Technique Bone Marrow Cells Platelet Membrane Glycoproteins In Vitro Techniques Biology Megakaryocyte medicine Humans Glycophorin Glycophorins education Cells Cultured Megakaryocytopoiesis Erythroid Precursor Cells education.field_of_study Hematology General Medicine Hematopoietic Stem Cells Phenotype Molecular biology Hematopoiesis Haematopoiesis medicine.anatomical_structure Immunology biology.protein Neoplastic cell Erythropoiesis Megakaryocytes Biomarkers |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1600-0609 0902-4441 |
| DOI: | 10.1111/j.1600-0609.1992.tb01804.x |
| Popis: | A population of haematopoietic cells co-expressing glycoprotein IIIa (GPIIIa), which has been shown to be present in the megakaryocyte-platelet lineage, and glycophorin A, which has been shown to be specific for the erythroid lineage, has been identified in normal bone marrow cultures using double immunofluorescence staining. The cells showing this phenotype have the size of lymphocytes and appear at an early time (day 1 to d 6) of culture. We have also detected a type of mixed megakaryocyte (MK) cluster containing this phenotype on d 3 or 4 in most normal marrow samples. Such a cell phenotype was not detected after 6 d of culture. It is thus the first time that this phenotype, although already described for several human neoplastic cell lines, has been observed in several normal human bone marrow cultures. Cells expressing this phenotype may represent haematopoietic cells, common to megakaryocytic and erythroid lineages, at a very early stage of differentiation. |
| Databáze: | OpenAIRE |
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