Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats
| Autor: | Zuhal Yildirim, Yusuf Çamlica, Ebru Gökalp Özkorkmaz, Coşar Uzun, Ayla Çelik, Meltem Kumaş, Yusuf Özay, Sevda Güzel, Gizem Güler, Mahmut Ülger, Ahmet Kahraman |
|---|---|
| Přispěvatelé: | KUMAŞ, Meltem, Uşak Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine DPPH Surgical Wound Wound healing Microbial Sensitivity Tests RM1-950 Salvia Antimicrobial activity Candida parapsilosis Antioxidants Diabetes Mellitus Experimental Ointments Candida tropicalis 03 medical and health sciences chemistry.chemical_compound Salvia kronenburgii 0302 clinical medicine medicine Animals Rats Wistar Skin Flavonoids Pharmacology Bacteria biology Traditional medicine Candida glabrata Salvia euphratica var. euphratica Plant Extracts Güzel S. Ozay Y. KUMAŞ M. Uzun C. Gökalp Özkorkmaz E. Yıldırım Z. Ülger M. Güler G. Çelik A. Çamlıca Y. et al. -Wound healing properties antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats- BIOMEDICINE & PHARMACOTHERAPY cilt.111 ss.1260-1276 2019 Broth microdilution Isoniazid Diabetes General Medicine biology.organism_classification Antimicrobial Anti-Bacterial Agents Rats Oxidative Stress 030104 developmental biology chemistry 030220 oncology & carcinogenesis Therapeutics. Pharmacology DNA Damage medicine.drug |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 111, Iss, Pp 1260-1276 (2019) |
| Popis: | PubMed ID: 30841440 Diabetic patients suffer from persistent and non-healing wounds. Salvia species are traditionally used for the treatment of wounds and colds. The aims of the present study were to evaluate the in vivo wound healing potential, in vitro antimicrobial and antioxidant activities, and total phenolic and flavonoid contents of the aerial parts of two endemic taxa, Salvia kronenburgii Rech. f. (SK) and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica (SE). Two different concentrations (0.5% and 1% (w/w)) of ethanol extracts were investigated in incision and excision wound models on Streptozotocin-induced diabetic rats using biomechanical, biochemical, histopathological, macroscopic, and genotoxic methods for 7 and 14 days. Antimicrobial activity was evaluated against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Acinetobacter baumannii, Aeromonas hydrophila, Mycobacterium tuberculosis, Candida glabrata, Candida parapsilosis, and Candida tropicalis using the broth microdilution and the resazurin microtiter assay plate methods. Fito ® , Ampicillin, Ethambutol, Isoniazid, and Fluconazole were used as reference drugs. Antioxidant capacities and total phenolic and flavonoid contents of both extracts were detected using DPPH free radical scavenging assay, Folin-Ciocalteu, and Al(NO 3 ) 3 methods, respectively. SK ointment at 0.5% and 1% (w/w) concentrations and SE ointment at 1% (w/w) concentration showed 99.9%, 99.5%, and 99.7% contraction, respectively for excision wounds, and SK and SE ointments at 1% (w/w) concentration showed 99.4% and 99.2% contraction for incision wounds while Fito ® showed 98.9% and 98.5% contraction, respectively. Increased re-epithelialization (P < 0.01 and P < 0.001), angiogenesis, and decreased dermal inflammation (P < 0.001) were determined for SK and SE ointments at both 7 and 14 days. SE ointment on day 7 and SK ointment on day 14 reduced oxidative damage to DNA when compared to control (P < 0.01 and P < 0.001). Both tested plants had greater antibacterial activity against A. baumannii (62.5 ?g/mL MIC value) and SE had greater antimycobacterial activity against M. tuberculosis (0.24 ?g/mL MIC value) when compared to reference drugs Ampicillin, Isoniazid, and Ethambutol (125, 0.97, and 1.95 ?g/mL MIC values, respectively). Antioxidant capacities, total phenolic and flavonoid contents of SE and SK were 87.08%, 76.21 ?g GAE/mg, 43.43 ?g QE/mg and 72.17%, 41.81 ?g GAE/mg, 33.62 ?g QE/mg, respectively. SK and SE had strong wound healing effects while SK found to be more effective than SE at both 7 and 14 days. © 2019 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|