Tissue reaction to subcutaneous implantation of a collagen sponge. A histological, ultrastructural, and immunological study
| Autor: | C. Bacques, Robert Garrone, Daniel J. Hartmann, Daniel Herbage, Guylène Charriere, Karine Anselme |
|---|---|
| Přispěvatelé: | Deleage, Gilbert, Laboratoire d'Histologie Expérimentale, URA 244, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Université de Lyon, Institut Pasteur de Lyon, Réseau International des Instituts Pasteur (RIIP), univOAK, Archive ouverte |
| Rok vydání: | 1990 |
| Předmět: |
Male
Surgical Sponges Pathology medicine.medical_specialty Biocompatibility Biomedical Engineering Biocompatible Materials Biology Biomaterials Materials Testing [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology medicine Animals Fibroblast [SDV.IB] Life Sciences [q-bio]/Bioengineering Inflammation Foreign-Body Reaction Soft tissue Biomaterial Histology Rats Inbred Strains Anatomy biology.organism_classification Rats Sponge Microscopy Electron medicine.anatomical_structure Ultrastructure [SDV.IB]Life Sciences [q-bio]/Bioengineering Implant Collagen |
| Zdroj: | HAL Journal of Biomedical Materials Research Journal of Biomedical Materials Research, John Wiley & Sons, 1990, 24 (6), pp.689-703. ⟨10.1002/jbm.820240605⟩ |
| ISSN: | 0021-9304 1097-4636 |
| DOI: | 10.1002/jbm.820240605⟩ |
| Popis: | The biocompatibility of a subcutaneously implanted bovine collagen sponge (Haemostagen) was studied in rats by analyzing tissue reactions up to 3 months by histological and ultrastructural methods; in addition, the level of serum antibodies to bovine type I collagen (the major implant collagen) was measured by solid-phase radioimmunoassay. By 8 h after implantation, the implant was completely filled with polymorphonuclear cells (PMNs). After 8 days, fibroblasts had developed a granulation tissue within the sponge and the PMNs had almost disappeared. The small residue that remained after 1 month consisted of some densely packed collagen fibrils containing giant cells, which had disappeared by 3 months. No antibodies to bovine type I collagen were found in the sera of implanted rats.The biocompatibility of a subcutaneously implanted bovine collagen sponge (Haemostagen) was studied in rats by analyzing tissue reactions up to 3 months by histological and ultrastructural methods; in addition, the level of serum antibodies to bovine type I collagen (the major implant collagen) was measured by solid-phase radioimmunoassay. By 8 h after implantation, the implant was completely filled with polymorphonuclear cells (PMNs). After 8 days, fibroblasts had developed a granulation tissue within the sponge and the PMNs had almost disappeared. The small residue that remained after 1 month consisted of some densely packed collagen fibrils containing giant cells, which had disappeared by 3 months. No antibodies to bovine type I collagen were found in the sera of implanted rats. |
| Databáze: | OpenAIRE |
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