Increased thymocyte differentiation in myasthenia gravis: A dual-color immunofluorescence phenotypic analysis
| Autor: | Caterina Casadio, U. Massazza, Luca Durelli, M. F. Ferrio, R. Cavallo, Giuliano Maggi, L. Bergamini, G. Poccardi, M. Di Summa |
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| Rok vydání: | 1990 |
| Předmět: |
Adult
Antigens Differentiation T-Lymphocyte Male medicine.medical_specialty medicine.drug_class CD1 Thymus Gland Biology Lymphocyte Activation Monoclonal antibody Immunofluorescence Antigen Internal medicine Myasthenia Gravis medicine Humans Aged medicine.diagnostic_test Middle Aged medicine.disease Immunohistochemistry Molecular biology Myasthenia gravis Thymocyte Phenotype Endocrinology Neurology Mature Thymocyte Common Thymocyte Female Neurology (clinical) |
| Zdroj: | Annals of Neurology. 27:174-180 |
| ISSN: | 1531-8249 0364-5134 |
| DOI: | 10.1002/ana.410270213 |
| Popis: | Thymocytes express multiple, different surface antigens according to their stage of maturation. Surface differentiation antigens have been studied with the technique of simultaneous dual-color, direct immunofluorescence in the thymuses of 20 patients with myasthenia gravis (MG) and 10 control subjects with cardiac diseases. Fluorescein isothiocyanate-conjugated and phycoerythrin-conjugated monoclonal antibodies were used to stain thymic cell suspensions. A significant decrease in the percentage of immature and common thymocyte phenotypes (CD1+,3+ and CD4+,8+) and a significant increase in the percentage of mature thymocyte phenotypes (CD1-,3+; CD4+,8-; and CD4-,8+) and of B cells (CD20+) were found in MG thymuses compared with controls. These data, indicating an increased availability of mature, fully immunocompetent T and B cells, indirectly suggest the occurrence of an active immune response in MG thymus. |
| Databáze: | OpenAIRE |
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