Achromobacter xylosoxidans Cellular Pathology Is Correlated with Activation of a Type III Secretion System
| Autor: | Maxx H. Tessmer, Aaron T. Dirck, Dara W. Frank, Nathan A. Ledeboer, Adam M. Pickrum, John R. Kirby, Molly O. Riegert, Orlando DeLeon, Julie Biller |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cytotoxicity
Immunologic 0301 basic medicine Cellular pathology Cystic Fibrosis Virulence Factors 030106 microbiology Immunology Virulence Biology Microbiology Cystic fibrosis Mice 03 medical and health sciences Tissue culture Bacterial Proteins Phagocytosis Cell Line Tumor Type III Secretion Systems medicine Animals Humans Secretion Cytotoxicity Gene Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Achromobacter xylosoxidans medicine.disease biology.organism_classification 030104 developmental biology Infectious Diseases Achromobacter denitrificans Host-Pathogen Interactions Cytokines Parasitology Inflammation Mediators Gram-Negative Bacterial Infections Biomarkers |
| Zdroj: | Infect Immun |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00136-20 |
| Popis: | Achromobacter xylosoxidans is increasingly recognized as a colonizer of cystic fibrosis (CF) patients, but the role that A. xylosoxidans plays in pathology remains unknown. This knowledge gap is largely due to the lack of model systems available to study the toxic potential of this bacterium. Recently, a phospholipase A(2) (PLA(2)) encoded by a majority of A. xylosoxidans genomes, termed AxoU, was identified. Here, we show that AxoU is a type III secretion system (T3SS) substrate that induces cytotoxicity to mammalian cells. A tissue culture model was developed showing that a subset of A. xylosoxidans isolates from CF patients induce cytotoxicity in macrophages, suggestive of a pathogenic or inflammatory role in the CF lung. In a toxic strain, cytotoxicity is correlated with transcriptional activation of axoU and T3SS genes, demonstrating that this model can be used as a tool to identify and track expression of virulence determinants produced by this poorly understood bacterium. |
| Databáze: | OpenAIRE |
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