Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean
| Autor: | Cinzia M. Bertea, Christian Garabello, Cristina Campobenedetto, Valeria Contartese, Giuseppe Mannino, Chiara Agliassa, Alberto Acquadro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant Glycine max medicine.medical_treatment seed germination Plant Science 01 natural sciences Article Ribosome assembly Transcriptome 03 medical and health sciences chemistry.chemical_compound biostimulant treatment medicine Food science RNA-Seq Incubation Ecology Evolution Behavior and Systematics Legume Biostimulant treatment ROS scavenging enzymes and molecules Seed germination Ecology fungi Botany food and beverages 030104 developmental biology chemistry Germination Seed treatment QK1-989 Glycine 010606 plant biology & botany |
| Zdroj: | Plants Plants, Vol 9, Iss 1308, p 1308 (2020) Volume 9 Issue 10 |
| ISSN: | 2223-7747 |
| Popis: | Soybean (Glycine max Merr.) is a worldwide important legume crop, whose growth and yield are negatively affected by heat stress at germination time. Here, we tested the role of a biostimulant based on lignin derivatives, plant-derived amino acids, and molybdenum in enhancing soybean heat stress tolerance when applied on seeds. After treatment with the biostimulant at 35 ° C, the seed biometric parameters were positively influenced after 24 h, meanwhile, germination percentage was increased after 72 h (+10%). RNA-Seq analyses revealed a modulation of 879 genes (51 upregulated and 828 downregulated) in biostimulant-treated seeds as compared with the control, at 24 h after incubation at 35 ° C. Surprisingly, more than 33% of upregulated genes encoded for ribosomal RNA (rRNA) methyltransferases and proteins involved in the ribosome assembly, acting in a specific protein network. Conversely, the downregulated genes were involved in stress response, hormone signaling, and primary metabolism. Finally, from a biochemical point of view, the dramatic H2O2 reduction 40%) correlated to a strong increase in non-protein thiols (+150%), suggested a lower oxidative stress level in biostimulant-treated seeds, at 24 h after incubation at 35 ° C. Our results provide insights on the biostimulant mechanism of action and on its application for seed treatments to improve heat stress tolerance during germination. |
| Databáze: | OpenAIRE |
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