Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications
| Autor: | Yu Jiang, Rania Oueslati, Shigetoshi Eda, Xiaogang Lin, Jayne Wu, Nan Wan, Jiamei Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Computer science
Point-of-Care Systems Capacitive sensing Point-of-care testing Femto Biosensing Techniques 02 engineering and technology TP1-1185 01 natural sciences Biochemistry Article Analytical Chemistry Electrokinetic phenomena Limit of Detection alternating current electrokinetic effects Electronic engineering Sensitivity (control systems) Electrical and Electronic Engineering Instrumentation miRNA sensing Point of care capacitive sensing Chemical technology 010401 analytical chemistry Response time Equipment Design 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences MicroRNAs 0210 nano-technology point-of-care diagnostics |
| Zdroj: | Sensors, Vol 21, Iss 3985, p 3985 (2021) Sensors Volume 21 Issue 12 Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| Popis: | A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|