Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview

Autor:	Brett Houser
Rok vydání:	2012
Předmět:	Analyte xMAP Physiology Nanotechnology Microsphere Sample volume Suspensions Physiology (medical) Chlorocebus aethiops Luminex medicine Animals Humans Multiplex Bio-Plex® Bio plex Immunoassay Chromatography multiplex detection medicine.diagnostic_test Flat surface Chemistry General Medicine Microspheres COS Cells Original Article ELISA
Zdroj:	Archives of Physiology and Biochemistry
ISSN:	1744-4160 1381-3455
DOI:	10.3109/13813455.2012.705301
Popis:	The Bio-Plex(®) system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods. Similar to ELISA, xMAP utilizes an antibody sandwich for detection but differs from ELISA in capture substrate and detection method. Rather than a flat surface, Bio-Plex(®) assays make use of differentially detectable bead sets as a substrate capturing analytes in solution and employs fluorescent methods for detection. These bead sets identify the analytes and detection antibodies are used to measure the quantity of analyte. The use of differentially detectable beads enables the simultaneous identification and quantification of many analytes in the same sample.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89bcf1282fa0aef67d020a28bc45f96e https://doi.org/10.3109/13813455.2012.705301 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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