Sanguineous normothermic, intermittent cardioplegia, effects on hypertrophic myocardium: morphometric, metabolic and ultrastructural studies in rabbits hearts
| Autor: | Valder Rodrigues de Mello, J E S Roselino, Clovis Carbone Junior, Paulo Roberto Barbosa Evora, A A Sader |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Coarctation of the aorta Myocardial Reperfusion Injury Mitochondrion Hypertrophy left ventricular Left ventricular hypertrophy Heart arrest induced Mitochondria Heart Statistics Nonparametric chemistry.chemical_compound Random Allocation Oxygen Consumption Glycogen breakdown Internal medicine Respiration medicine Animals Cardiovascular surgical procedures Glycogen business.industry Myocardium Body Weight Heart General Medicine Organ Size Cardiomyopathy Hypertrophic medicine.disease Respiratory quotient Disease Models Animal chemistry Ultrastructure Cardiology Heart Arrest Induced Surgery Rabbits Cardiology and Cardiovascular Medicine business |
| Zdroj: | Brazilian Journal of Cardiovascular Surgery v.27 n.4 2012 Brazilian Journal of Cardiovascular Surgery Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) instacron:SBCCV |
| Popis: | OBJECTIVES: The present investigation aimed to study the protective effect of intermittent normothermic cardioplegia in rabbit's hypertrophic hearts. METHODS: The parameters chosen were 1) the ratio heart weight / body weight, 2) the myocardial glycogen levels, 3) ultrastructural changes of light and electron microscopy, and 4) mitochondrial respiration. RESULTS: 1) The experimental model, coarctation of the aorta induced left ventricular hypertrophy; 2) the temporal evolution of the glycogen levels in hypertrophic myocardium demonstrates that there is a significant decrease; 3) It was observed a time-dependent trend of higher oxygen consumption values in the hypertrophic group; 4) there was a significant time-dependent decrease in the respiratory coefficient rate in the hypertrophic group; 5) the stoichiometries values of the ADP: O2 revealed the downward trend of the values of the hypertrophic group; 6) It was possible to observe damaged mitochondria from hypertrophic myocardium emphasizing the large heterogeneity of data. CONCLUSION: The acquisition of biochemical data, especially the increase in speed of glycogen breakdown, when anatomical changes are not detected, represents an important result even when considering all the difficulties inherent in the process of translating experimental results into clinical practice. With regard to the adopted methods, it is clear that morphometric methods are less specific. Otherwise, the biochemical data allow detecting alterations of glycogen concentrations and mitochondria respiration before the morphometric alterations should be detected |
| Databáze: | OpenAIRE |
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