Adipose-Derived Regenerative Cells Promote Tendon-Bone Healing in a Rabbit Model
| Autor: | Hiroyuki Tsuchiya, Masahiro Kosaka, Junsuke Nakase, Katsuhiro Hayashi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Anterior cruciate ligament reconstruction medicine.medical_treatment Anterior cruciate ligament Adipose tissue Fibrin Tissue Adhesive Regenerative Medicine Bone tissue Tendons 03 medical and health sciences 0302 clinical medicine medicine Animals Regeneration Orthopedics and Sports Medicine Fibrin glue 030222 orthopedics Anterior Cruciate Ligament Reconstruction business.industry Therapeutic effect Adipose-Derived Regenerative Cells 030229 sport sciences musculoskeletal system Stifle Biomechanical Phenomena Surgery medicine.anatomical_structure Adipose Tissue Models Animal Tissue Adhesives Rabbits business Stem Cell Transplantation |
| Zdroj: | Arthroscopy: The Journal of Arthroscopic & Related Surgery. 32:851-859 |
| ISSN: | 0749-8063 |
| DOI: | 10.1016/j.arthro.2015.10.012 |
| Popis: | Purpose To evaluate the therapeutic effect of adipose-derived regenerative cell (ADRC) administration on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model. Methods ACL reconstruction with semitendinosus tendon autograft was performed in the right knees of adult white rabbits. Eighty rabbits were divided into 2 groups: the treatment group, in which the graft was coated with ADRCs mixed in a fibrin glue carrier during surgery, and the control group, in which the graft was coated with fibrin glue only. At 2, 4, 6, 8, and 12 weeks postoperatively, 8 rabbits were killed in each group. Three were used for histologic evaluation at the tendon-bone interface and 5 for biomechanical examination. Results On histologic analysis, chondroid cells appeared more orderly and more regular in size and shape and Sharpey-like fibers, which connected the tendon graft and bone tissue, appeared earlier in ADRC-treated tissues than in control tissues. On biomechanical analysis, the ultimate failure load in the ADRC-treated group was significantly greater than that in the control group at 2 weeks (29.5 ± 7.2 N v 20.9 ± 2.7 N, P = .016) and 4 weeks (32.3 ± 3.9 N v 22.8 ± 5.4 N, P = .016). Stiffness was significantly higher in the ADRC-treated group than in the control group at 6 weeks (21.7 ± 5.9 N/mm v 12.6 ± 4.9 N/mm, P = .037). Although the ultimate failure load and stiffness of the ADRC-treated limbs were higher than those of the limbs in the control group at 8 and 12 weeks, these differences were not significant. Conclusions Local administration of ADRCs promoted the early healing process at the tendon-bone junction, both histologically and mechanically, in a rabbit ACL reconstruction model. Clinical Relevance ADRCs could be used to enhance graft healing in ACL reconstruction. |
| Databáze: | OpenAIRE |
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