Expression of perforin on HIV-1-specific CD8+ lymphocytes after immunization with a gp120-depleted, whole-killed HIV-1 immunogen
| Autor: | Dennis J. Carlo, R. Z. Maigetter, Giermakowska Wk, Fred C. Jensen, Ronald B. Moss, Mark R. Wallace |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Immunogen Anti-HIV Agents medicine.medical_treatment Lymphocyte Immunology chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Immune system HIV Seropositivity medicine Humans Immunology and Allergy Cytotoxic T cell AIDS Vaccines Membrane Glycoproteins biology Perforin Vaccination virus diseases Immunity to Infection T lymphocyte Immunotherapy Flow Cytometry Virology medicine.anatomical_structure Immunization HIV-1 biology.protein T-Lymphocytes Cytotoxic |
| Zdroj: | Clinical and Experimental Immunology. 124:248-254 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2001.01534.x |
| Popis: | SUMMARYWe examined HIV-1 antigen specific intracellular expression of perforin on CD4+ and CD8+ lymphocytes in subjects with chronic HIV-1 infection on antiviral drug therapy after immunization with a gp120-depleted, whole killed HIV-1 immunogen (inactivated, gp120-depleted HIV-1 in IFA, Remune). Based upon previous results, we hypothesized that the restoration of adequate T helper immune responses by vaccination against HIV-1 could result in the augmentation of CD8+ lymphocyte immune responses measured as perforin expression. In the current study we observed an increase in the frequency of perforin in CD8+ lymphocytes in HIV infected individuals immunized with a gp120-depleted HIV-1 immunogen while on antiviral drug therapy. Furthermore, the frequency of HIV-specific CD8+ perforin expressing cells correlated with the T helper immune response as measured by the lymphocyte proliferative response (LPR). The induction of such responses with immunization may have direct antiviral consequences and is being studied inm ongoing clinical trials. |
| Databáze: | OpenAIRE |
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