Split-QF system for fine-tuned transgene expression in Drosophila
| Autor: | Richard A. Baines, Samuel W. Vernon, Barry J. Dickson, Olena Riabinina |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Genetics
0303 health sciences animal structures biology Transgene quinic acid Quinic acid biology.organism_classification Expression (mathematics) Cell biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry split-LexA split-GAL4 Drosophila (subgenus) Q system q-system 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Genetics, 2019, Vol.212(1), pp.53-63 [Peer Reviewed Journal] Riabinina, O, Vernon, S, Dickson, B J & Baines, R 2019, ' Split-QF system for fine-tuned transgene expression in Drosophila ', Genetics . https://doi.org/10.1534/genetics.119.302034 Genetics |
| Popis: | The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|