Development and Validation of HPLC Method for the Determination of Pregabalin in Capsules
| Autor: | Mazahar Farooqui, Gajanan B. Kasawar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
validation
Chromatography Phosphate buffered saline Method development Pregabalin Analytical chemistry Short Communications Pharmaceutical Science Linearity Reversed-phase chromatography Capsule Dosage Form chemistry.chemical_compound chemistry Phase (matter) RP-HPLC medicine pregabalin Acetonitrile Hplc method medicine.drug |
| Zdroj: | Indian Journal of Pharmaceutical Sciences |
| ISSN: | 1998-3743 0250-474X |
| Popis: | A simple, precise, specific, and accurate reverse phase HPLC method has been developed for the determination of pregabalin in capsule dosage form. The chromatography was set on Hypersil BDS, C8, 150×4.6 mm, 5 μm column using photodiode array detector. The mobile phase consisting of phosphate buffer pH 6.9 and acetonitrile in the ratio of 95:05 with flow rate of 1 ml/min. The method was validated according to ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. Lower limit of quantification is 0.6 mg/l. The pregabalin sample solution was found to be stable at room temperature for about 26 h. |
| Databáze: | OpenAIRE |
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