Terahertz Dynamics in Human Cells and Their Chromatin
| Autor: | Mario Calvitti, M. Marconi, Irene Pirisinu, Giulio Monaco, Federico Sebastiani, Alessandro Paciaroni, Caterina Petrillo, Andrea Orecchini, Rita Romani, Marco Zanatta, M. Longo, Francesco Sacchetti |
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| Rok vydání: | 2014 |
| Předmět: |
collective dynamics
inelastic X-ray scattering nuclear DNA uranyl staining X-ray diffraction Materials Science (all) inelastic x-ray scattering Scattering Terahertz radiation Dynamics (mechanics) Nanotechnology Biology Molecular physics Chromatin Superposition principle Speed of sound Dispersion (optics) chromatin General Materials Science Physical and Theoretical Chemistry Line (formation) |
| Zdroj: | The journal of physical chemistry letters 5 (2014): 2177–2181. doi:10.1021/jz500918w info:cnr-pdr/source/autori:Longo, M.; Marconi, M.; Orecchini, A.; Petrillo, C.; Monaco, G.; Calvitti, M.; Pirisinu, I.; Romani, R.; Sacchetti, F.; Sebastiani, F.; Zanatta, M.; Paciaroni, A./titolo:Terahertz Dynamics in Human Cells and Their Chromatin/doi:10.1021%2Fjz500918w/rivista:The journal of physical chemistry letters/anno:2014/pagina_da:2177/pagina_a:2181/intervallo_pagine:2177–2181/volume:5 |
| DOI: | 10.1021/jz500918w |
| Popis: | The terahertz dynamics of human cells of the U937 line and their chromatin has been investigated by high-resolution inelastic X-ray scattering. To highlight its dynamical features in situ, nuclear DNA has been stained by uranyl-acetate salt. The general behavior of the collective dynamics of the whole cell is quite similar to that of bulk water, with a nearly wavevector-independent branch located at about 5 meV and a propagating mode with a linear trend corresponding to a speed of sound of 2900 +/- 100 m/s. We provide the first experimental evidence for the existence of two branches also in the dispersion curves of chromatin. The high-energy mode displays an acoustic-like behavior with a sound velocity similar to unstained cells, but in this case the branch likely originates from the superposition of intramolecular DNA optic modes. A low-energy optic-like branch, distinctive of the chromatin moiety, is found at about 2.5 meV. |
| Databáze: | OpenAIRE |
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