Differential Changes in Endothelin-1 and Endothelial Nitric Oxide Synthase Gene Expression in Rats with a Chronic High Pulmonary Flow
| Autor: | Ing-Jun Chen, Zen-Kong Dai, Chu-Chong Lu, Jiunn-Ren Wu, Mian-Shin Tan, Chee-Yin Chai, Aij-Lie Kwan, Arco Y. Jeng, Sheng-Long Howng |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pulmonary Circulation medicine.medical_specialty Time Factors Nitric Oxide Synthase Type III Transcription Genetic Nitric Oxide Synthase Type II Pulmonary Artery Biology Gene Expression Regulation Enzymologic Arteriovenous Shunt Surgical Downregulation and upregulation Western blot Internal medicine medicine.artery Gene expression medicine Animals Aorta Abdominal RNA Messenger Protein Precursors Rats Wistar Pharmacology Messenger RNA Lung Endothelin-1 medicine.diagnostic_test Endothelin 1 Rats Surgery Endocrinology medicine.anatomical_structure Blood pressure Pulmonary artery Venae Cavae Cardiology and Cardiovascular Medicine |
| Zdroj: | Journal of Cardiovascular Pharmacology. 44:S341-S345 |
| ISSN: | 0160-2446 |
| DOI: | 10.1097/01.fjc.0000166266.09550.a7 |
| Popis: | The expressions of endothelin-1 (ET-1) and endothelial nitric oxide synthase were assessed in the lung of adult Wistar rats (n = 6/group) undergoing an abdominal aortocaval shunt to increase pulmonary blood flow for 4, 8 or 12 weeks. The shunt resulted in significant medial hypertrophy of the pulmonary artery without significant increases in pulmonary and systemic arterial pressure. A competitive reverse transcriptase-polymerase chain reaction demonstrated significant increases in pulmonary preproET-1 mRNA at 12 weeks (mean +/- standard error of the mean; shunt, 1.82 +/- 0.12; sham, 1.00 +/- 0.15; P < 0.05) and in pulmonary endothelial nitric oxide synthase mRNA at both 8 weeks (shunt, 1.57 +/- 0.12; sham, 1.00 +/- 0.18; P < 0.05) and 12 weeks (shunt, 1.89 +/- 0.18; sham, 1.00 +/- 0.13; P < 0.05). In addition, western blot analysis showed increases in pulmonary endothelial nitric oxide synthase protein by 126% and 164% at 8 and 12 weeks, respectively, in the shunt animals. However, the plasma ET-1 concentrations and the lung ET-1 contents were unchanged. These results indicate that endothelial nitric oxide synthase gene expression was upregulated, prior to that of ET-1, at the transcriptional level during pulmonary vascular remodeling in this chronic shunt model. |
| Databáze: | OpenAIRE |
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