Inverse correlation between Naa10p and Pirh2 expression and the combined prognostic value in oral squamous cell carcinoma patients
| Autor: | Gang Du, Wang Fazhan, Yuhao Liu, Jun Zheng, Yongyong Yang, Yan Zeng, Jiang Xu, Keying Wang, Jinhua Yang |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Oncology Cancer Research medicine.medical_specialty Multivariate statistics Ubiquitin-Protein Ligases Biology Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Western blot Internal medicine Biomarkers Tumor medicine Humans Basal cell N-Terminal Acetyltransferase E RNA Messenger Inverse correlation N-Terminal Acetyltransferase A Survival analysis Messenger RNA medicine.diagnostic_test Univariate 030206 dentistry Middle Aged Prognosis Immunohistochemistry stomatognathic diseases Otorhinolaryngology 030220 oncology & carcinogenesis Carcinoma Squamous Cell Periodontics Female Mouth Neoplasms Oral Surgery |
| Zdroj: | Journal of Oral Pathology & Medicine. 48:686-695 |
| ISSN: | 1600-0714 0904-2512 |
| Popis: | Background This study aims to explore the associations between N-α-acetyltransferase 10 protein (Naa10p) and p53-induced protein with a RING-H2 domain (Pirh2) expression and clinicopathological characteristics in oral squamous cell carcinoma (OSCC). Methods Immunohistochemistry was performed to detect Naa10p and Pirh2 levels containing 118 OSCC specimens, and additional analyses were used to determine correlations between Naa10p and Pirh2 expressions, generate survival curves, and perform univariate and multivariate statistical analyses. Further, quantitative real-time PCR (qRT-PCR) and western blot were employed to examine Naa10p and Pirh2 expression level in OSCC patients' samples. We further validated the result using RNAseq data from The Cancer Genome Atlas (TCGA) and mRNA array data from GSE31056 and GSE30784. Results Naa10p and Pirh2 are overexpression, and the protein level of Naa10p was negatively correlated with that of Pirh2 in OSCC tissues. Multivariate Cox proportional hazard regression analysis showed that positive Naa10p expression and negative Pirh2 expression were both independent good prognostic factors for OSCC patients. Furthermore, the Naa10p-positive/Pirh2-negative group has the best prognosis among all OSCC patients. Results from qRT-PCR showed the higher expression level of Naa10 and lower expression level of Pirh2 in tumor tissues than adjacent normal tissues. TCGA database and data from GSE31056 and GSE30784 showed the similar result. The correlation analysis showed that the mRNA level of Naa10 was negatively correlated that of Pirh2. Conclusion The expression of Naa10p is negatively correlated with that of Pirh2, and positive Naa10p and negative Pirh2 might be independent biomarkers for better OSCC prognoses. |
| Databáze: | OpenAIRE |
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