Silica nanoparticles doped with anthraquinone for lung cancer phototherapy
| Autor: | Josué S. Bello Forero, Rodrigo J. Corrêa, Nanci C. de Lucas, Emerson Schwingel Ribeiro, Daniela Dias Queiroz, Ronaldo Custodio de Souza Oliveira, Eric C. Romani, Simon J. Garden, Raquel Simas Pereira Teixeira, Rodrigo Da Silva Souza, Marcos D. Pereira |
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| Rok vydání: | 2016 |
| Předmět: |
Lung Neoplasms
medicine.medical_treatment Biophysics Nanoparticle Anthraquinones Photodynamic therapy 02 engineering and technology Adenocarcinoma 010402 general chemistry Photochemistry 01 natural sciences Anthraquinone chemistry.chemical_compound Microscopy Electron Transmission Cell Line Tumor Spectroscopy Fourier Transform Infrared Rose bengal medicine Humans Radiology Nuclear Medicine and imaging Drug Carriers Rose Bengal Photosensitizing Agents Radiation Radiological and Ultrasound Technology Singlet oxygen Silicon Dioxide 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Covalent bond Microscopy Electron Scanning Nanoparticles Flash photolysis Spectrophotometry Ultraviolet 0210 nano-technology Phototoxicity |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 165:1-9 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2016.10.008 |
| Popis: | In the present study, SiO2 nanoparticles functionalized with 3-(2-aminoethylamino)propyl group (SiNP-AAP) were used, for the first time, to covalently bond rose bengal (SiNP-AAP-RB) or 9,10-anthraquinone-2-carboxylic acid (SiNP-AAP-OCAq). The functionalized SiNP were characterized by: Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM); elemental analysis (CHN) for determination of the dye concentration; FTIR and UV-vis diffuse reflectance (DR-UV-vis) and a surface area study (BET). The functionalized SiNPs were applied in photodynamic therapy (PDT) against lung cancer cell lines. The evaluated cytotoxicity revealed 20-30% cell survival after 15min of PDT for both materials but the OCAq concentration was half of the RB nanomaterial. The phototoxicity was mainly related to oxidative stress generated in the cellular environment by singlet oxygen and by hydrogen abstraction as confirmed by the laser flash photolysis technique. The unprecedented results indicate that SiNP-AAP-OCAq is a possible system for promoting cell apoptosis by both type I and type II mechanisms. |
| Databáze: | OpenAIRE |
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