Corrigendum: Sho1 and Msb2 Play Complementary but Distinct Roles in Stress Responses, Sexual Differentiation, and Pathogenicity of Cryptococcus neoformans
| Autor: | Juyeong Jang, Michal A. Olszewski, Yee Seul So, Yong Sun Bahn, Jintao Xu, Goun Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Saccharomyces cerevisiae Mutant lcsh:QR1-502 Virulence C. neoformans Microbiology lcsh:Microbiology 03 medical and health sciences HOG mucin Original Research Cryptococcus neoformans biology Endoplasmic reticulum Correction biology.organism_classification Transmembrane protein Cell biology mating 030104 developmental biology Phosphorylation osmotic stress Function (biology) |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 9 (2018) Frontiers in Microbiology, Vol 11 (2020) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2020.01956 |
| Popis: | The high-osmolarity glycerol response (HOG) pathway is pivotal in environmental stress response, differentiation, and virulence of Cryptococcus neoformans, which causes fatal meningoencephalitis. A putative membrane sensor protein, Sho1, has been postulated to regulate HOG pathway, but its regulatory mechanism remains elusive. In this study, we characterized the function of Sho1 with relation to the HOG pathway in C. neoformans. Sho1 played minor roles in osmoresistance, thermotolerance, and maintenance of membrane integrity mainly in a HOG-independent manner. However, it was dispensable for cryostress resistance, primarily mediated through the HOG pathway. A mucin-like transmembrane (TM) protein, Msb2, which interacts with Sho1 in Saccharomyces cerevisiae, was identified in C. neoformans, but found not to interact with Sho1. MSB2 codeletion with SHO1 further decreased osmoresistance and membrane integrity, but not thermotolerance, of sho1Δ mutant, indicating that both factors play to some level redundant but also discrete roles in C. neoformans. Sho1 and Msb2 played redundant roles in promoting the filamentous growth in sexual differentiation in a Cpk1-independent manner, in contrast to the inhibitory effect of the HOG pathway in the process. However, both factors contributed independently to Cpk1 phosphorylation during vegetative growth and endoplasmic reticulum (ER) stress response. Finally, Sho1 and Msb2 play distinct but complementary roles in the pulmonary virulence of C. neoformans. Overall, Sho1 and Msb2 play complementary but distinct roles in stress response, differentiation, and pathogenicity of C. neoformans. |
| Databáze: | OpenAIRE |
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