Effect of vitamin A in enteral formulae for burned guinea-pigs
| Autor: | K. Kuroiwa, Jeffrey L. Nelson, Jean Tchervenkov, J. Wesley Alexander, S. Inoue, O. Trocki |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Vitamin
Staphylococcus aureus medicine.medical_specialty Calorie Guinea Pigs Nutritional Status Critical Care and Intensive Care Medicine Enteral administration Random Allocation chemistry.chemical_compound Enteral Nutrition Internal medicine Animals Medicine Hypersensitivity Delayed Vitamin A Body surface area business.industry Liter General Medicine medicine.disease Hypervitaminosis A Vitamin A deficiency Disease Models Animal Red blood cell Endocrinology medicine.anatomical_structure chemistry Emergency Medicine Dinitrofluorobenzene Female Surgery Burns business human activities |
| Zdroj: | Burns. 16:265-272 |
| ISSN: | 0305-4179 |
| DOI: | 10.1016/0305-4179(90)90137-l |
| Popis: | A burned guinea-pig model (30 per cent body surface area) was used to study the effects of dietary vitamin A. Sixty-five female guinea-pigs were infused enterally via gastrostomy feeding tubes with identical formulae (175 kcal/kg/day, 20 per cent of calories as protein) containing varying amounts of vitamin A. Groups I, II, III and IV received formulae containing 0, 10 000iu (approximately equivalent to the guinea-pigs' RDA), 50 000 iu (5 x RDA) and 250 000 iu (25 x RDA) of vitamin A per litre, respectively. After 14 days of tube feeding, the animals were killed. Group I animals had evidence of vitamin A deficiency including low haemoglobin levels, lower red blood cell counts and lower caecal mucosal weight. Findings of hypervitaminosis A were observed only in animals given the highest dose of vitamin A (25 × RDA). These were elevated serum alkaline phosphatase and complement C3 levels and enlarged adrenal glands. Group IV also showed defective cell-mediated immunity as reflected by reduced delayed cutaneous response to dinitrofluorobenzene. In a second experiment groups I, II, III and IV were given formulas containing 0,1 × RDA, 5 × RDA, and 10 × RDA of vitamin A respectively for 14 days. Through postburn days 12 to 14 they were injected subcutaneously with 3 × 108 of Staphylococcus aureus once daily. On postburn day 15 the animals were killed and the numbers of viable bacteria at each injection site were counted. No significant differences were observed in viable bacterial numbers between the groups. Serum vitamin A concentration was significantly lower in group I, and there was no sign of hypervitaminosis A in the other groups. These findings suggest that vitamin A is needed following burn injury, and doses of I RDA to 10 RDA may be administered safely. However, overdosage (25 × RDA) may be quite harmful. |
| Databáze: | OpenAIRE |
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