Multicolor fate mapping of Langerhans cell homeostasis
| Autor: | Hervé Luche, Bernard Malissen, Stephan Wienert, Marc Bajénoff, Jonathan A. Nowak, Sonja Zahner, Clément Ghigo, Bjoern E. Clausen, Audrey Jorquera, Frederick Klauschen, Isabelle Mondor |
|---|---|
| Přispěvatelé: | Immunology |
| Rok vydání: | 2013 |
| Předmět: |
Langerhans cell
Cell division Immunology Population Cell Cytological Techniques Color Mice Transgenic Biology 03 medical and health sciences Mice 0302 clinical medicine Immune system Imaging Three-Dimensional Fate mapping medicine Immunology and Allergy Animals Homeostasis Cell Lineage education 030304 developmental biology Inflammation 0303 health sciences education.field_of_study Epidermis (botany) integumentary system Brief Definitive Report Cell biology Mice Inbred C57BL medicine.anatomical_structure 030220 oncology & carcinogenesis Langerhans Cells Stem cell |
| Zdroj: | Journal of Experimental Medicine, 210(9), 1657-1664. Rockefeller University Press The Journal of Experimental Medicine Europe PubMed Central |
| ISSN: | 0022-1007 |
| DOI: | 10.1084/jem.20130403 |
| Popis: | The adult epidermal Langerhans cell network is formed by adjacent proliferative units composed of dividing cells and their terminally differentiated daughter cells. Langerhans cells (LCs) constitute a network of immune sentinels in the skin epidermis that is seeded during embryogenesis. Whereas the development of LCs has been extensively studied, much less is known about the homeostatic renewal of adult LCs in “nonmanipulated” animals. Here, we present a new multicolor fluorescent fate mapping system and quantification approach to investigate adult LC homeostasis. This novel approach enables us to propose and provide evidence for a model in which the adult epidermal LC network is not formed by mature coequal LCs endowed with proliferative capabilities, but rather constituted by adjacent proliferative units composed of “dividing” LCs and their terminally differentiated daughter cells. Altogether, our results demonstrate the general utility of our novel fate-mapping system to follow cell population dynamics in vivo and to establish an alternative model for LC homeostasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|