Detecting AhR ligands in sediments using bioluminescent reporter yeast
| Autor: | Tereza Šídlová, Piia Pessala, Marko Virta, Klára Hilscherová, Simo Salo, Piia Leskinen, Hannu Kiviranta, Matti Verta |
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| Rok vydání: | 2008 |
| Předmět: |
Geologic Sediments
Luminescence Biomedical Engineering Biophysics Saccharomyces cerevisiae 010501 environmental sciences Ligands 01 natural sciences 03 medical and health sciences Electrochemistry Soil Pollutants Bioassay Bioluminescence 030304 developmental biology 0105 earth and related environmental sciences Pollutant 0303 health sciences biology Ligand Equipment Design General Medicine Aryl hydrocarbon receptor Yeast Equipment Failure Analysis Receptors Aryl Hydrocarbon Biochemistry 13. Climate action Environmental chemistry Luminescent Measurements biology.protein Biological Assay Bioreporter Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 23:1850-1855 |
| ISSN: | 0956-5663 |
| Popis: | Sediments polluted with high concentrations of persistent organic pollutants, many of which are ligands of the aryl hydrocarbon receptor (AhR), are currently of concern around the industrialized world. Bioassays that can detect the presence of AhR ligands in environmental samples offer a relatively rapid and cost-effective means of prioritizing samples before more elaborate, laborious, and costly chemical analyses are applied. This paper presents a new bioluminescent yeast assay based on transcriptional activation of AhR. Its applicability for determining AhR ligands in complex environmental samples was demonstrated by analyzing a set of sediment samples from the River Kymi, Finland. The results from the assay are shown to be consistent with those from both a chemical analysis and an H4IIE-luc bioassay. The yeast assay procedure is simple and can be performed within 1 day. The yeasts grow rapidly, are easy to handle, and do not require continuous cell culturing. Moreover, the robustness of the yeast allows the application of the test to crude extracts or even sediment suspensions. The yeast assay described in this paper can be useful in screening and prioritization of samples prior to chemical analysis. Moreover, the strain can be used in the construction of fibre-optic biosensors. |
| Databáze: | OpenAIRE |
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