Haemocompatibility improvement of metallic surfaces by covalent immobilization of heparin–liposomes
| Autor: | Michail Kastellorizios, Georgios P. A. K. Michanetzis, P. Favia, Ricardo d’Agostino, Pavlos G. Klepetsanis, Spyridon Mourtas, Sophia G. Antimisiaris, Bianca Rita Pistillo, Eloisa Sardella, Yannis F. Missirlis |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Plasma processing
Pharmaceutical Science Polyethylene Glycols Plasma chemistry.chemical_compound Platelet Adhesiveness Platelet adhesiveness Stent medicine Humans Surface charge Particle Size Acrylic acid Liposome Heparin Chemistry Phosphatidylethanolamines Vesicle technology industry and agriculture Anticoagulants Stainless Steel Cholesterol Biochemistry Metals Covalent bond Drug delivery Liposomes Phosphatidylcholines Haemocompatibility Nuclear chemistry medicine.drug |
| Zdroj: | International journal of pharmaceutics 432 (2012): 91–98. doi:10.1016/j.ijpharm.2012.04.057 info:cnr-pdr/source/autori:Kastellorizios M, Michanetzis GPAK, Pistillo BR, Mourtas S, Klepetsanis P, Favia P, Sardella E, d'Agostino R, Missirlis YF, Antimisiaris SG/titolo:Haemocompatibility improvement of metallic surfaces by covalent immobilization of heparin-liposomes/doi:10.1016%2Fj.ijpharm.2012.04.057/rivista:International journal of pharmaceutics (Print)/anno:2012/pagina_da:91/pagina_a:98/intervallo_pagine:91–98/volume:432 |
| ISSN: | 0378-5173 |
| Popis: | Stainless steel surfaces were processed by means of plasma enhanced chemical vapor deposition (PE-CVD) fed with acrylic acid vapors in order to functionalize them with carboxyl groups, which were subsequently activated for covalent immobilization of heparin-loaded (HEP) NH2 group-functionalized (Fun) nanoliposomes (NLs). Empty Fun or HEP non-functionalized (control) NLs were used as controls. NLs were characterized for mean diameter, surface charge and heparin encapsulation/release. Different lipid compositions were used for NL construction; PC/Chol (2:1 mol/mol) or PC/Chol (4:1 mol/mol) (fluid type vesicles) [ which allow gradual release of heparin] and DSPC/Chol (2:1 mol/mol) (rigid type vesicles). Surface haemocompatibility was tested by measuring blood clotting time. Platelet adhesion on surfaces was evaluated morphologically by SEM and CLSM. The haemocompatibility of plasma-processed surfaces was improved (compared to untreated surfaces); Fun-HEP NL-coated surfaces demonstrated highest coagulation times. For short surface/blood incubation periods, surfaces coated with Fun-HEP NLs consisting of PC/Chol (2:1) had higher coagulation times (compared to DSPC/Chol NLs) due to faster release of heparin. Heparin release rate from the various NL types and surface platelet adhesion results were in agreement with the corresponding blood coagulation times. Concluding, covalent immobilization of drug entrapping NLs on plasma processed surfaces is a potential method for preparation of controlled-rate drug-eluting metallic stents or devices. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect