Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus
| Autor: | Gulsin Arslan, Sinan Alkan, Serdar Karakurt, Idris Sargin |
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| Rok vydání: | 2021 |
| Předmět: |
Mushroom
Fluorophore Sociology and Political Science biology Chemistry Clinical Biochemistry Quantum yield biology.organism_classification Pleurotus Biochemistry Fluorescence Carbon Edible mushroom Clinical Psychology chemistry.chemical_compound Live cell imaging Quantum Dots Pleurotus ostreatus Law Spectroscopy Social Sciences (miscellaneous) Agaricus bisporus Nuclear chemistry |
| Zdroj: | Journal of fluorescence. 31(5) |
| ISSN: | 1573-4994 |
| Popis: | In the study, fluorescent imaging of live cells was performed using fluorescent carbon quantum dots derived from edible mushrooms species; Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus as a fluorophore agent. Carbon quantum dots were synthesized through a facile and low-cost method based on microwave irradiation of dried mushroom samples in hydrogen peroxide solution under optimized conditions (microwave energy, solution type, duration of microwave treatment, amount of mushroom). Upon purification with centrifugation, microfiltration, and dialysis, the lyophilized carbon quantum dots were identified through UV-visible, fluorescence and FT-IR, X-ray photoelectron spectroscopy, X-ray diffraction, high-resolution transmission electron microscopy, and quantum yield calculation. Cell viability assessment of the carbon quantum dots was evaluated against human epithelial cell line PNT1A using the Alamar Blue Assay. In vitro fluorescence cell imaging studies demonstrated that the carbon dots could dynamically penetrate the cell membrane and nuclear membrane and localize in both the cytoplasm and the nucleus. |
| Databáze: | OpenAIRE |
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