‘Candidatus Phytoplasma solani’ interferes with the distribution and uptake of iron in tomato
Autor: | Fabio Marroni, Marta Martini, Rita Musetti, Sara Buoso, Laura Pagliari, Simonetta Santi, Wolfgang Schmidt |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
Chlorophyll Leaves Chloroplasts Plant Roots 01 natural sciences Carotenoids metabolism chemistry.chemical_compound Solanum lycopersicum Photosynthesis Iron deficiency NGS Phloem Phytoplasma Porphyrin Roots Tomato 2. Zero hunger 0303 health sciences Chlorosis biology food and beverages Shoot Research Article Biotechnology lcsh:QH426-470 Iron lcsh:Biotechnology Flowers 03 medical and health sciences lcsh:TP248.13-248.65 Botany Genetics Candidatus Phytoplasma solani Iron deficiency (plant disorder) Plant Diseases 030304 developmental biology Gene Expression Profiling fungi Biological Transport biology.organism_classification Plant Leaves lcsh:Genetics chemistry Acholeplasmataceae Solanum 010606 plant biology & botany |
Zdroj: | BMC Genomics, Vol 20, Iss 1, Pp 1-21 (2019) BMC Genomics |
ISSN: | 1471-2164 |
Popis: | Background ‘Candidatus Phytoplasma solani’ is endemic in Europe and infects a wide range of weeds and cultivated plants. Phytoplasmas are prokaryotic plant pathogens that colonize the sieve elements of their host plant, causing severe alterations in phloem function and impairment of assimilate translocation. Typical symptoms of infected plants include yellowing of leaves or shoots, leaf curling, and general stunting, but the molecular mechanisms underlying most of the reported changes remain largely enigmatic. To infer a possible involvement of Fe in the host-phytoplasma interaction, we investigated the effects of ‘Candidatus Phytoplasma solani’ infection on tomato plants (Solanum lycopersicum cv. Micro-Tom) grown under different Fe regimes. Results Both phytoplasma infection and Fe starvation led to the development of chlorotic leaves and altered thylakoid organization. In infected plants, Fe accumulated in phloem tissue, altering the local distribution of Fe. In infected plants, Fe starvation had additive effects on chlorophyll content and leaf chlorosis, suggesting that the two conditions affected the phenotypic readout via separate routes. To gain insights into the transcriptional response to phytoplasma infection, or Fe deficiency, transcriptome profiling was performed on midrib-enriched leaves. RNA-seq analysis revealed that both stress conditions altered the expression of a large (> 800) subset of common genes involved in photosynthetic light reactions, porphyrin / chlorophyll metabolism, and in flowering control. In Fe-deficient plants, phytoplasma infection perturbed the Fe deficiency response in roots, possibly by interference with the synthesis or transport of a promotive signal transmitted from the leaves to the roots. Conclusions ‘Candidatus Phytoplasma solani’ infection changes the Fe distribution in tomato leaves, affects the photosynthetic machinery and perturbs the orchestration of root-mediated transport processes by compromising shoot-to-root communication. |
Databáze: | OpenAIRE |
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