Identification of Urinary Metabolites of Orally AdministeredN,N-Dimethyl-p-Toluidine in Male F344 Rats∗
| Autor: | Katayoon Ghanbari, Nam-Cheol Kim, Waylon Weber, Jacob D. McDonald, Kelly J. Dix, Dean Kracko |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Magnetic Resonance Spectroscopy Chromatography Toluidines Health Toxicology and Mutagenesis Metabolite Extraction (chemistry) Administration Oral Hippuric acid Metabolism Toxicology Mass spectrometry High-performance liquid chromatography Mass Spectrometry Rats Inbred F344 Rats chemistry.chemical_compound chemistry Animals Carbon Radioisotopes Gas chromatography Chromatography High Pressure Liquid Demethylation |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 70:781-788 |
| ISSN: | 1087-2620 1528-7394 |
| Popis: | The metabolism of orally administered N,N-dimethyl-p-toluidine (DMPT) in male F344 rats was investigated. The rat urinary metabolite profile was determined by analytical reverse-phase high performance liquid chromatography (HPLC). Four radiolabeled peaks were observed, isolated, and purified by solid-phase extraction (SPE) and preparative HPLC methods. The 4 peaks were identified as p-(N-acetylhydroxyamino)hippuric acid (M1), DMPT N-oxide (M2), N-methyl-p-toluidine (M3), and parent DMPT. Metabolites M1 and M2 were identified by spectrometric and spectroscopic methods, including mass fragmentation pattern identification from both liquid chromatography/mass spectrometry and gas chromatography/mass spectrometry, and from chemical analysis of nuclear magnetic resonance spectra. Structural confirmation of metabolite M2 was accomplished by comparison with a synthetic standard. Peaks M3 and the peak suspected to be DMPT were identified by comparison of their HPLC retention times and mass fragmentation patterns with authentic standards of N-methyl-p-toluidine and DMPT, respectively. DMPT metabolism is similar to that reported for N,N-dimethylaniline. |
| Databáze: | OpenAIRE |
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