MS-based metabolomics revealing Bornean Sinularia sp. extract dysregulated lipids triggering programmed cell death in Hepatocellular carcinoma
| Autor: | Perng Yang Puah, Yee Soon Ling, Leong Rui Lim, Oriana Tamin, Yoong Soon Yong |
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| Rok vydání: | 2020 |
| Předmět: |
Programmed cell death
010405 organic chemistry Organic Chemistry Cancer Plant Science Biology medicine.disease biology.organism_classification 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry 010404 medicinal & biomolecular chemistry Metabolomics Cell culture Hepatocellular carcinoma medicine Cancer research Carcinoma Sinularia |
| DOI: | 10.6084/m9.figshare.7524848 |
| Popis: | Soft coral, Sinularia sp. had been proven to inherit promising anti-cancer properties against variety of cancer. Current study, Sinularia sp. extract was introduced to Hepatocellular carcinoma (Hep 3B). Cell viability assay indicated the extract exhibit a dose and time dependent cytotoxicity. LC50 exhibited the lowest at 72 h post treatment estimated as 45.3 µg/mL. Morphological alterations including nuclear condensation, cytoplasm shrinkage and deformed cellular shape in treated Hep 3B were observable. Chemometric analysis revealed hydrophobic metabolites were significantly altered. Elevated vitamin D and derivatives tend to up-regulation Ca2+ and ROS subsequently triggering apoptosis. Dysregulated glycerolipids may suggest that they were biotransformed to compensate the needs of phospholipids during cell damage. Perturbation of sphingolipids, ceramide and carbohydrate-conjugated ceramides species increased the release of pro-apoptotic components reside within mitochondria and promote programmed cell death in treated Hep 3B. To conclude, MS-based metabolomics enabled the characterization of Sinularia sp. extract-induced cell death. |
| Databáze: | OpenAIRE |
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