Fast Magnetic Field-Enhanced Linear Colloidal Agglutination Immunoassay
| Autor: | Jean-Philippe Gineys, Gilles Cauet, Jean Baudry, Jérôme Bibette, Nevzat Temurok, Aurélien Daynes, Philippe Nerin |
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| Přispěvatelé: | HORIBA Medical (HORIBA ABX SAS), HORIBA Scientific [France], Laboratoire Colloïdes et Matériaux Divisés (LCMD), Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL) |
| Rok vydání: | 2015 |
| Předmět: |
Immunoassay
Spectrum analyzer Dose-Response Relationship Drug medicine.diagnostic_test Chemistry Pulse (signal processing) Orders of magnitude (temperature) Analytical chemistry equipment and supplies Analytical Chemistry Magnetic field Kinetics Magnetics Colloid Acceleration Agglutination (biology) C-Reactive Protein Limit of Detection medicine Humans Colloids [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] human activities |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2015, 87 (15), ⟨10.1021/acs.analchem.5b00279⟩ |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.5b00279 |
| Popis: | International audience; We present the principle of a fast magnetic field enhanced colloidal agglutination assay, which is based on the acceleration of the recognition rate between ligands and receptors induced by magnetic forces.1 By applying a homogeneous magnetic field of 20 mT for only 7 s, we detect CRP (C-reactive protein) in human serum at a concentration as low as 1 pM for a total cycle time of about 1 min in a prototype analyzer. Such a short measurement time does not impair the performances of the assay when compared to longer experiments. The concentration range dynamic is shown to cover 3 orders of magnitude. An analytical model of agglutination is also successfully fitting our data obtained with a short magnetic pulse. |
| Databáze: | OpenAIRE |
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