Characterization and quantitation of yohimbine and its analogs in botanicals and dietary supplements using LC/QTOF-MS and LC/QQQ-MS for determination of the presence of bark extract and yohimbine adulteration
| Autor: | James Neal-Kababick, Jerry Zweigenbaum, Derick Lucas |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Food Contamination
Quechers Mass Spectrometry Analytical Chemistry Lc qtof ms chemistry.chemical_compound medicine Environmental Chemistry Pharmacology Indole test Lc qqq ms Ajmalicine Chromatography Molecular Structure Plant Extracts Yohimbine Triple quadrupole mass spectrometer chemistry visual_art Dietary Supplements visual_art.visual_art_medium Plant Bark Bark Plant Preparations Drug Contamination Agronomy and Crop Science Food Science medicine.drug Chromatography Liquid |
| Zdroj: | Journal of AOAC International. 98(2) |
| ISSN: | 1060-3271 |
| Popis: | The compound yohimbine HCl has been restricted in Australia and categorized as a scheduled prescription drug in other parts of the world, including the United States where it is monographed as a drug in the U. S. Pharmacopeia. However, the bark of the yohimbe plant and its extract is considered a botanical that can be used as a dietarysupplement in some partsof the world. For these reasons, methods to characterize the indole alkaloids of the bark and quantify yohimbine and its analogs are presented using accurate mass LC/quadrupole time-of-flight (QTOF)-MS and triple quadrupole LC/MS, respectively. Samples were extracted with a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method to characterize and quantify the indole alkaloids. With the LC/QTOF-MS in auto MS/MS mode the indole alkaloids were identified,and the isomeric response of each could be used to determine whether the actual bark or extract was in samples of dietary supplements and not adulteration with yohimbine HCl. Analogs were identified and include yohimbic acid, methyl yohimbine, and hydroxyl yohimbine. Many isomers of each were also detected, butidentified only by the number of chromatographic peaks. Quantification of yohimbine and ajmalicine spiked extracts showed recoveries of 99 to 103% with RSD of 3.6% or lower and LODs of less than 100 ppt. Calibration of the two standards gave r2 = 0.9999 in a range from 0.1 to 100 ppb. Dietary supplements quantified for these two compounds showed a range from not detected to 3x the amounts found in thebark. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|