In vitrotoxicity of functionalised nanoclays is mainly driven by the presence of organic modifiers
| Autor: | Gemma Vilar, David González-Gálvez, Socorro Vázquez-Campos, Elisabet Fernández-Rosas, Gemma Janer, Carmen López-Iglesias, Ezequiel Mas del Molino, Valentina Ermini |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Materials science
Cell Survival Biomedical Engineering Apoptosis Toxicology chemistry.chemical_compound Cell Line Tumor Humans Organoclay Organic chemistry Ammonium Viability assay Particle Size Cytotoxicity Analysis of Variance In vitro Culture Media Nanostructures Montmorillonite chemistry Toxicity Bentonite Clay Aluminum Silicates Particle size Nuclear chemistry |
| Zdroj: | Nanotoxicology. 8:279-294 |
| ISSN: | 1743-5404 1743-5390 |
| DOI: | 10.3109/17435390.2013.776123 |
| Popis: | Little information exists on the toxicological hazards associated to organo-modified clays. We evaluated the cytotoxicity of a series of pristine and organo-modified nanoclays in different cell lines. The calculated IC50 values for cell viability ranged from 1.4 to 47 µg/mL for the six organoclays used and were above 100 µg/mL for the pristine nanoclays. The IC50 values of the organoclays were driven by the proportion and structure of the quaternary ammonium compound used as surface organic modifier. No differences in cell toxicity were observed between the large and small-sized (additional milling step) nanoclay batches, although their size differences related mostly to upper range of the size distribution. Despite their lower toxicity, pristine nanoclays induced apoptosis and were found in cytoplasmic vesicles of exposed cells. Organoclays were also found in cytoplasmic vesicles, although the size of the agglomerates was larger and the efficiency of uptake was considerably lower. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|