The genomic study of an environmental isolate of Scedosporium apiospermum shows its metabolic potential to degrade hydrocarbons
| Autor: | Martha J. Vives, Laura Isabel Tolosa Morales, Laura Natalia González-García, María Cecilia O’Byrne Orozco, Silvia Restrepo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genome lcsh:QH426-470 030106 microbiology Xylene Hydrocarbon degradation Sequence assembly Scedosporium apiospermum Biology lcsh:Genetics 03 medical and health sciences chemistry.chemical_compound Metabolic pathway 030104 developmental biology Biochemistry chemistry Genetics Microbial biodegradation Extended Genome Report Gene DNA |
| Zdroj: | Standards in Genomic Sciences Standards in Genomic Sciences, Vol 12, Iss 1, Pp 1-12 (2017) |
| ISSN: | 1944-3277 |
| DOI: | 10.1186/s40793-017-0287-6 |
| Popis: | Crude oil contamination of soils and waters is a worldwide problem, which has been actively addressed in recent years. Sequencing genomes of microorganisms involved in the degradation of hydrocarbons have allowed the identification of several promoters, genes, and degradation pathways of these contaminants. This knowledge allows a better understanding of the functional dynamics of microbial degradation. Here, we report a first draft of the 44.2 Mbp genome assembly of an environmental strain of the fungus Scedosporium apiospermum. The assembly consisted of 178 high-quality DNA scaffolds with 1.93% of sequence repeats identified. A total of 11,195 protein-coding genes were predicted including a diverse group of gene families involved in hydrocarbon degradation pathways like dioxygenases and cytochrome P450. The metabolic pathways identified in the genome can potentially degrade hydrocarbons like chloroalkane/alkene, chorocyclohexane, and chlorobenzene, benzoate, aminobenzoate, fluorobenzoate, toluene, caprolactam, geraniol, naphthalene, styrene, atrazine, dioxin, xylene, ethylbenzene, and polycyclic aromatic hydrocarbons. The comparison analysis between this strain and the previous sequenced clinical strain showed important differences in terms of annotated genes involved in the hydrocarbon degradation process. Electronic supplementary material The online version of this article (10.1186/s40793-017-0287-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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