Long Noncoding RNA Lnc-SEMT Modulates IGF2 Expression by Sponging miR-125b to Promote Sheep Muscle Development and Growth
| Autor: | Shijin Hu, Jiafan Liu, Chuduan Wang, Caihong Wei, Xiaogang Wang, Mingxing Chu, Zhen Liu, Li Yang, Ruizao Liu, Shuzhen Zhang, Yayu Wang, Zehu Yuan, Huijing Zhao, Lixin Du, Mingming Wu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
myoblast differentiation Physiology Transgene medicine.medical_treatment Biology Muscle Development MyoD lcsh:Physiology miR-125b lcsh:Biochemistry Animals Genetically Modified Myoblasts 03 medical and health sciences Insulin-Like Growth Factor II medicine Animals Myocyte lcsh:QD415-436 RNA Small Interfering 3' Untranslated Regions Cells Cultured MyoD Protein Sheep lcsh:QP1-981 Base Sequence Myoglobin Growth factor IGF2 Antagomirs RNA Skeletal muscle Cell Differentiation sheep muscularity Phenotype LncRNA Long non-coding RNA Cell biology MicroRNAs 030104 developmental biology medicine.anatomical_structure RNA Interference RNA Long Noncoding Sequence Alignment |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 49, Iss 2, Pp 447-462 (2018) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: Long noncoding RNAs (lncRNAs) are RNA transcripts that are more than 200 nt long but have little protein-coding potential. Within the last few years, thousands of lncRNAs have been identified and their functions in biological processes have begun to be understood. Although many studies havebegun to examine the functions of many noncoding RNAs, very little is known about the functions of long noncoding (lncRNA) function of livestock production and molecular mechanisms of their functions are still lackingrelated to livestock production. Methods: Expression of sheep enhanced muscularityTranscript lncRNA (lnc-SEMT) and miR-125b were examined in sheep using quantitative reverse-transcription polymerase chain reaction. Expression of Myod (myogenic determination factor), Myog (myoglobin) and Insulin-like growth factor 2 (IGF2)were examined by Western Blot.Luciferase reporter assays were performedto confirm the relationship between lnc-SEMT and miR-125b. Results: Here, we identified a novel lnc-SEMT that promote sheep myoblast differentiation in vitro and enhanced sheep muscularity in vivo. Functional analyses showed that lnc-SEMT accelerates sheep myoblast differentiation in vitro. lnc-SEMT transgenic sheep exhibit a muscle hypertrophy phenotype characterized by increased body weight, and increased the number of muscle fibers indicating that lnc-SEMT play an important role in the regulation of skeletal muscle differentiation in vivo. Our results show that lnc-SEMT acts as a molecular sponge by antagonizing miR-125b to control IGF2 protein labundance in vitro and in vivo. Conclusion: In brief, lnc-SEMT is the first example of a lncRNA could be a useful candidate for improving biological growth traits such as skeletal muscle production in sheep. |
| Databáze: | OpenAIRE |
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