Anorexic effect of K+ channel blockade in mesenteric arterial smooth muscle and intestinal epithelial cells
| Autor: | Michele Sweeney, Sharon S. McDaniel, Stefanie Krick, Jason X.-J. Yuan, Bethany R. Lapp, Oleksandr Platoshyn, Jian-Ying Wang, Vera A. Golovina, Victor A. Miriel, Ying Yu |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Patch-Clamp Techniques Potassium Channels Physiology Biology Muscle Smooth Vascular Membrane Potentials Rats Sprague-Dawley Physiology (medical) Internal medicine Isometric Contraction Appetite Depressants medicine Potassium Channel Blockers Animals 4-Aminopyridine Cells Cultured Membrane potential Reverse Transcriptase Polymerase Chain Reaction Body Weight Depolarization Epithelial Cells Voltage-gated potassium channel Epithelium Blockade Mesenteric Arteries Rats Electrophysiology Intestines medicine.anatomical_structure Endocrinology Calcium medicine.symptom Vasoconstriction Artery |
| Zdroj: | Journal of applied physiology (Bethesda, Md. : 1985). 91(5) |
| ISSN: | 8750-7587 |
| Popis: | Activity of voltage-gated K+(Kv) channels controls membrane potential ( Em). Membrane depolarization due to blockade of K+channels in mesenteric artery smooth muscle cells (MASMC) should increase cytoplasmic free Ca2+concentration ([Ca2+]cyt) and cause vasoconstriction, which may subsequently reduce the mesenteric blood flow and inhibit the transportation of absorbed nutrients to the liver and adipose tissue. In this study, we characterized and compared the electrophysiological properties and molecular identities of Kv channels and examined the role of Kv channel function in regulating Emin MASMC and intestinal epithelial cells (IEC). MASMC and IEC functionally expressed multiple Kv channel α- and β-subunits (Kv1.1, Kv1.2, Kv1.3, Kv1.4, Kv1.5, Kv2.1, Kv4.3, and Kv9.3, as well as Kvβ1.1, Kvβ2.1, and Kvβ3), but only MASMC expressed voltage-dependent Ca2+channels. The current density and the activation and inactivation kinetics of whole cell Kv currents were similar in MASMC and IEC. Extracellular application of 4-aminopyridine (4-AP), a Kv-channel blocker, reduced whole cell Kv currents and caused Emdepolarization in both MASMC and IEC. The 4-AP-induced Emdepolarization increased [Ca2+]cytin MASMC and caused mesenteric vasoconstriction. Furthermore, ingestion of 4-AP significantly reduced the weight gain in rats. These results suggest that MASMC and IEC express multiple Kv channel α- and β-subunits. The function of these Kv channels plays an important role in controlling Em. The membrane depolarization-mediated increase in [Ca2+]cytin MASMC and mesenteric vasoconstriction may inhibit transportation of absorbed nutrients via mesenteric circulation and limit weight gain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|