Characterization of Nda2, a Plastoquinone-reducing Type II NAD(P)H Dehydrogenase in Chlamydomonas Chloroplasts
| Autor: | Carine Desplats, Emmanuelle Billon, Florence Mus, Laurent Cournac, Gilles Peltier, Stéphan Cuiné |
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| Přispěvatelé: | Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Bioénergie et Microalgues (EBM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
Plastoquinone [SDV]Life Sciences [q-bio] Protozoan Proteins Chlamydomonas reinhardtii Dehydrogenase Photosystem I 01 natural sciences Biochemistry Thylakoids Electron Transport 03 medical and health sciences chemistry.chemical_compound NAD(P)H dehydrogenase Escherichia coli Animals Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Chlamydomonas Genetic Complementation Test NADPH Dehydrogenase Cell Biology Chlororespiration biology.organism_classification Recombinant Proteins chemistry Thylakoid Genome Protozoan Oxidation-Reduction NADP 010606 plant biology & botany Hydrogen |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, 2009, 284 (7), pp.4148-4157. ⟨10.1074/jbc.M804546200⟩ Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (7), pp.4148-4157. ⟨10.1074/jbc.M804546200⟩ |
| ISSN: | 0021-9258 1083-351X |
| Popis: | Electron transfer pathways associated to oxygenic photosynthesis, including cyclic electron flow around photosystem I and chlororespiration, rely on non-photochemical reduction of plastoquinones (PQs). In higher plant chloroplasts, a bacterial-like NDH complex homologous to complex I is involved in PQ reduction, but such a complex is absent from Chlamydomonas plastids where a type II NAD(P)H dehydrogenase activity has been proposed to operate. With the aim to elucidate the nature of the enzyme-supporting non-photochemical reduction of PQs, one of the type II NAD(P)H dehydrogenases identified in the Chlamydomonas reinhardtii genome (Nda2) was produced as a recombinant protein in Escherichia coli and further characterized. As many type II NAD(P)H dehydrogenases, Nda2 uses NADH as a preferential substrate, but in contrast to the eukaryotic enzymes described so far, contains non-covalently bound FMN as a cofactor. When expressed at a low level, Nda2 complements growth of an E. coli lacking both NDH-1 and NDH-2, but is toxic at high expression levels. Using an antibody raised against the recombinant protein and based on its mass spectrometric identification, we show that Nda2 is localized in thylakoid membranes. Chlorophyll fluorescence measurements performed on thylakoid membranes show that Nda2 is able to interact with thylakoid membranes of C. reinhardtii by reducing PQs from exogenous NADH or NADPH. We discuss the possible involvement of Nda2 in cyclic electron flow around PSI, chlororespiration, and hydrogen production. |
| Databáze: | OpenAIRE |
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