Spontaneous Protein Adsorption on Graphene Oxide Nanosheets Allowing Efficient Intracellular Vaccine Protein Delivery
| Autor: | Bruno G. De Geest, Lorenzo Albertazzi, Hui Li, Thomas De Beer, Riet De Rycke, Nane Vanparijs, Stefaan De Koker, Ans De Beuckelaer, Kaat Fierens, Katleen Van Steendam, Dieter Deforce, Johan Grooten, Zhiyue Zhang, Bart N. Lambrecht, Martijn J. Schuijs, Natàlia Feiner Gracia |
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| Přispěvatelé: | Supramolecular Polymer Chemistry |
| Rok vydání: | 2016 |
| Předmět: |
nanosheets
Materials science T-Lymphocytes Graphite oxide Nanotechnology 02 engineering and technology SDG 3 – Goede gezondheid en welzijn 010402 general chemistry 01 natural sciences chemistry.chemical_compound Drug Delivery Systems SDG 3 - Good Health and Well-being Antigen Humans General Materials Science dendritic cells Antigen-presenting cell Nanosheet Bioconjugation Proteins Cross-presentation Oxides vaccines 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences chemistry Biophysics graphene oxide Graphite Adsorption protein 0210 nano-technology Intracellular Protein adsorption |
| Zdroj: | ACS Applied Materials & Interfaces, 8(2), 1147-1155. American Chemical Society |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Nanomaterials hold potential of altering the interaction between therapeutic molecules and target cells or tissues. High aspect ratio nanomaterials in particular have been reported to possess unprecedented properties and are intensively investigated for their interaction with biological systems. Graphene oxide (GOx) is a water-soluble graphene derivative that combines high aspect ratio dimension with functional groups that can be exploited for bioconjugation. Here, we demonstrate that GOx nanosheets can spontaneously adsorb proteins by a combination of interactions. This property is then explored for intracellular protein vaccine delivery, in view of the potential of GOx nanosheets to destabilize lipid membranes such as those of intracellular vesicles. Using a series of in vitro experiments, we show that GOx nanosheet adsorbed proteins are efficiently internalized by dendritic cells (DCs: the most potent class of antigen presenting cells of the immune system) and promote antigen cross-presentation to CD8 T cells. The latter is a hallmark in the induction of potent cellular antigen-specific immune responses against intracellular pathogens and cancer. |
| Databáze: | OpenAIRE |
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