PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids
| Autor: | E. Gerhart H. Wagner, Millicent Masters, Fredrik Söderbom, Lin He, Nigel Binns, Uta Binnie |
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| Rok vydání: | 1993 |
| Předmět: |
Polyadenylation
Transcription Genetic Recombinant Fusion Proteins Molecular Sequence Data Colicins Biology Microbiology Polymerase Chain Reaction Plasmid Bacterial Proteins RNA interference Escherichia coli Polyadenylate RNA Antisense Molecular Biology DNA Primers ColE1 Base Sequence Escherichia coli Proteins fungi Polynucleotide Adenylyltransferase Blotting Northern Molecular biology Fusion protein Antisense RNA Kinetics RNA Bacterial Genes Bacterial Primer (molecular biology) Ampicillin Resistance Plasmids |
| Zdroj: | Molecular microbiology. 9(6) |
| ISSN: | 0950-382X |
| Popis: | The replication of ColE1-related plasmids is controlled by an unstable antisense RNA, RNAI, which can interfere with the successful processing of the RNAII primer of replication. We show here that a host protein, PcnB, supports replication by promoting the decay of RNAI. In bacterial strains deleted for PcnB a stable, active form of RNAI, RNAI*, which appears to be identical to the product of 5'-end processing by RNAase E, accumulates. This leads to a reduction in plasmid copy number. We show, using a GST-PcnB fusion protein, that PcnB does not interfere with RNAI/RNAII binding in vitro. The fusion protein, like PcnB, has polyadenylating activity and is able to polyadenylate RNAI (and also another antisense RNA, CopA) in vitro. |
| Databáze: | OpenAIRE |
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