1H, 13C and 15N resonance assignments of σS activating protein Crl from Salmonella enterica serovar Typhimurium

Autor: Christina Sizun, Françoise Norel, Paola Cavaliere
Přispěvatelé: Systèmes macromoléculaires et Signalisation, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the French National Research Agency (Grant ANR-11-BSV3-009) and the IR-RMN-THC (CNRS FR3050), ANR-11-BSV3-0009,SIGMADAPT,Rôle de SigmaS dans la compétitivité et l'adaptation des bactéries à l'environnement(2011), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Proton Magnetic Resonance Spectroscopy
Molecular Sequence Data
Sigma factor
MESH: Sequence Alignment
MESH: Amino Acid Sequence
Biology
medicine.disease_cause
Biochemistry
Microbiology
MESH: Proteus mirabilis/metabolism
chemistry.chemical_compound
Bacterial Proteins
RpoS
Structural Biology
Transcription (biology)
RNA polymerase
MESH: Sigma Factor/metabolism
medicine
MESH: Carbon-13 Magnetic Resonance Spectroscopy
Amino Acid Sequence
Carbon-13 Magnetic Resonance Spectroscopy
Proteus mirabilis
Transcription factor
Gene
Escherichia coli
MESH: Proton Magnetic Resonance Spectroscopy
MESH: Molecular Sequence Data
Nitrogen Isotopes
Stress response
fungi
food and beverages
MESH: Salmonella typhimurium/metabolism
biology.organism_classification
Cell biology
Crl
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
chemistry
Salmonella enterica
Salmonella Typhimurium
Sequence Alignment
rpoS
MESH: Bacterial Proteins/chemistry
MESH: Nitrogen Isotopes
Zdroj: Biomolecular NMR Assignments
Biomolecular NMR Assignments, 2015, 9 (2), pp.397-401. ⟨10.1007/s12104-015-9617-z⟩
Biomolecular NMR Assignments, Springer, 2015, 9 (2), pp.397-401. ⟨10.1007/s12104-015-9617-z⟩
ISSN: 1874-270X
DOI: 10.1007/s12104-015-9617-z⟩
Popis: The general stress response in Enterobacteria, like Escherichia coli or Salmonella, is controlled by the transcription factor σS, encoded by the rpoS gene, which accumulates during stationary phase growth and associates with the core RNA polymerase enzyme (E) to promote transcription of genes involved in cell survival. Tight regulation of σS is essential to preserve the balance between self-preservation under stress conditions and nutritional competence in the absence of stress. Whereas σ factors are generally inactivated upon interaction with anti-sigma proteins, σS binding by the Crl protein facilitates the formation of the holoenzyme EσS, and therefore σS-controlled transcription. Previously, critical residues in both Crl and σS were identified and assigned to the binding interface in the Crl–σS complex. However, high-resolution structural data are missing to fully understand the molecular mechanisms underlying σS activation by Crl, in particular the possible role of Crl in triggering domain rearrangements in the multi-domain protein σS. Here we provide the 1H, 13C and 15N resonance assignments of Salmonella enterica serovar Typhimurium Crl, as a starting point for CrlSTM structure determination and further structural investigation of the CrlSTM–σ STM S complex.
Databáze: OpenAIRE
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