Phenotypic detection of beta-lactamases production in enterobacteriaceae
| Autor: | Katarina Kostic, Ljiljana Pavlovic, Slobodanka Djukic, Neda Konstantinović, Snezana Jovanovic, Ivana Cirkovic |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.drug_class
Klebsiella pneumoniae Antibiotics lcsh:Medicine medicine.disease_cause Microbiology beta-lactamases resistance 03 medical and health sciences MICROBIOLOGY PROCEDURES Enterobacteriaceae Drug Resistance Bacterial Escherichia coli medicine Humans Agar diffusion test 030304 developmental biology 2. Zero hunger 0303 health sciences biology 030306 microbiology business.industry lcsh:R General Medicine biology.organism_classification Proteus mirabilis 3. Good health Phenotype beta-Lactamase Inhibitors business Serbia enterobacteria Bacteria |
| Zdroj: | Srpski Arhiv za Celokupno Lekarstvo, Vol 142, Iss 7-8, Pp 457-463 (2014) Srpski arhiv za celokupno lekarstvo (2014) 142(7-8):457-463 Srpski arhiv za celokupno lekarstvo |
| ISSN: | 2406-0895 0370-8179 |
| DOI: | 10.2298/sarh1408457c |
| Popis: | Introduction. Beta-lactam antibiotics are the most commonly used antibacterial drugs. However, many bacteria have developed resistance to these antibiotics, and the most common form of resistance is the production of beta-lactamase enzymes. Many members of the Enterobacteriaceae family produce different types of these enzymes. Objective. The aim of this study was to perform phenotypic detection of production and identification of beta-lactamase type in Enterobacteriaceae isolated from different clinical specimens from patients hospitalized in the Clinical Center of Serbia. Methods. The strains of Enterobacteriaceae were collected between November 2011 and January 2012 in the laboratory of the Clinical Center of Serbia. The isolates were identified according to the standard microbiology procedures and confirmed by the Vitek2 automated system. Antimicrobial susceptibility testing was performed by the disk diffusion method, and the phenotypic detection of production and identification of betalactamases was performed according to previously described methodologies. Results. In this study, a total of 172 Enterobacteriaceae strains were isolated. Further testing was performed on 54/145 (37.2%) strains showing decreased susceptibility to beta-lactam antibiotics: 13/85 (15.3%) Escherichia coli, 31/46 (67.4%) Klebsiella pneumoniae and 10/14 (71.4%) Proteus mirabilis. Among them, 40/145 (27.6%) strains produced extended spectrum betalactamases (ESBLs), 9/145 (6.2%) - AmpC, 1/145 (0.7%) - K1 beta-lactamase and 4/145 (2.8%) - carbapenemases. Carbapenemases were predominantly detected in K. pneumoniae (75%). Conclusion. Enterobacteriaceae produce different types of betalactamases, and the most common type in our study was ESBLs. Production of carbapenemases detected in Enterobacteriaceae is also an associated problem. [Projekat Ministarstva nauke Republike Srbije, br. ON 175039] |
| Databáze: | OpenAIRE |
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