Vibrio tapetis from wrasse used for ectoparasite bio-control in salmon farming: phylogenetic analysis and serotyping
| Autor: | Henning Sørum, Duncan J. Colquhoun, Snorre Gulla, Øyvind Vågnes, Anita Rønneseth, Jesús L. Romalde, Sabela Balboa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Fish farming Pilot Projects Wrasse Ectoparasitic Infestations Aquatic Science Cleaner fish Labrus bergylta Microbiology Copepoda Fish Diseases 03 medical and health sciences Aquaculture Salmon louse Animals Serotyping Phylogeny Ecology Evolution Behavior and Systematics Vibrio Vibrio tapetis biology Phylogenetic tree business.industry Infection trial Fishes Biocontrol 04 agricultural and veterinary sciences biology.organism_classification 030104 developmental biology Biological Control Agents Lepeophtheirus Multilocus sequence analysis 040102 fisheries 0401 agriculture forestry and fisheries business |
| Zdroj: | Diseases of Aquatic Organisms |
| ISSN: | 1616-1580 0177-5103 |
| Popis: | So-called ‘cleaner fish’, including various wrasse (Labridae) species, have become increasingly popular in Norwegian salmon farming in recent years for biocontrol of the salmon louse Lepeophtheirus salmonis. Cleaner fish mortalities in salmon farms are, however, often high. Various bacterial agents are frequently associated with episodes of increased cleaner fish mortality, and Vibrio tapetis is regularly cultured from diseased wrasse. In the present study, we investigated the genetic relationships among 54 V. tapetis isolates (34 from wrasse species) by multilocus sequence analysis (MLSA; rpoD, ftsZ, pyrH, rpoA and atpA). In the resulting phylogenetic tree, all wrasse isolates belonged to sub-clusters within V. tapetis subsp. tapetis. Slide agglutination testing further confirmed the complete dominance amongst these isolates of 4 O-antigen serotypes, designated here as V. tapetis subsp. tapetis serotypes O1, O3, O4 and O5, respectively. A pilot challenge trial using serotypes O3, O4 and O5 did not indicate high pathogenicity towards ballan wrasse Labrus bergylta, thus questioning the role of V. tapetis as a primary pathogen of this fish species. publishedVersion |
| Databáze: | OpenAIRE |
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