How to Overcome the Disturbing Effects of Human Anti-Mouse Antibodies (Hama) on in Vitro Assays
| Autor: | K N Lema, Gustav Hör, B. Donnerstag, J.B. Oltrogge, Richard P. Baum |
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| Rok vydání: | 1997 |
| Předmět: |
0301 basic medicine
Cancer Research Antibodies Neoplasm medicine.drug_class Clinical Biochemistry Monoclonal antibody Chromatography Affinity Pathology and Forensic Medicine Immunoscintigraphy Mice 03 medical and health sciences 0302 clinical medicine Ovarian carcinoma Biomarkers Tumor medicine Animals Humans False Positive Reactions Tumor marker Ovarian Neoplasms Immunoradiometric assay biology Antibodies Monoclonal Radioimmunoassay Antibodies Anti-Idiotypic 030104 developmental biology Oncology CA-125 Antigen 030220 oncology & carcinogenesis Immunology biology.protein Female Immunoradiometric Assay Antibody Protein A Follow-Up Studies |
| Zdroj: | The International Journal of Biological Markers. 12:15-17 |
| ISSN: | 1724-6008 |
| Popis: | The development of human anti-mouse antibodies (HAMA) is a common immune response in patients with ovarian carcinoma after repeated injections of murine anti-CA 125 monoclonal antibodies for immunoscintigraphy. As a tumor marker with significant diagnostic value CA 125 is routinely measured in the follow-up of tumor patients by immunoradiometric assays (IRMA) based on murine anti-CA 125 monoclonal antibodies. HAMA may cause false-positive findings in a CA 125-IRMA. In this report our group demonstrates a simple way of eliminating HAMA by protein A/G affinity chromatography allowing the reliable detection of the tumor marker CA 125 in the serum of patients with ovarian carcinoma. |
| Databáze: | OpenAIRE |
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