Didelphis marsupialis (common opossum): a potential reservoir host for zoonotic leishmaniasis in the metropolitan region of Belo Horizonte (Minas Gerais, Brazil)
| Autor: | Célia Maria Ferreira Gontijo, Gerard J. Schoone, Henk D. F. H. Schallig, Eduardo Silva, Wendy F. van der Meide |
|---|---|
| Přispěvatelé: | KIT: Biomedical Research, Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Veterinary medicine
Disease reservoir Didelphis Antibodies Protozoan Biology Microbiology law.invention Seroepidemiologic Studies law Opossum Agglutination Tests Zoonoses Virology parasitic diseases medicine Animals Fluorescent Antibody Technique Indirect Leishmaniasis Polymerase chain reaction Disease Reservoirs Leishmania Leishmania chagasi DNA Protozoan biology.organism_classification medicine.disease Leishmania braziliensis Infectious Diseases Population Surveillance Restriction fragment length polymorphism Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Vector borne and zoonotic diseases (Larchmont, N.Y.), 7(3), 387-393. Mary Ann Liebert Inc. |
| ISSN: | 1530-3667 |
| Popis: | Identification of the zoonotic reservoir is important for leishmaniasis control program. A number of (wild) animal species may serve as reservoir hosts, including the opossum Didelphis marsupialis. A survey carried out in Didelphis specimens (n = 111) from the metropolitan region of Belo Horizonte, an important focus of human leishmaniasis in Brazil, is reported. All animals were serologically tested with indirect fluorescence antibody test (IFAT) and direct agglutination tests (DAT) based on L. (L.) donovani or L. (V.) braziliensis antigen. A sub-population (n = 20) was analyzed with polymerase chain reaction (PCR) for the presence of Leishmania-specific DNA. For species identification, PCR-positive samples were subjected to restriction enzyme fragment polymorphism (RFLP) analysis. Depending on the sero-diagnostic test employed, the sero-prevalence varied between 8.1% (9/111 animals positive with DAT test based on L. braziliensis antigen) and 21.6% (24/111 animals positive with IFAT). Five out of 20 samples analyzed with PCR tested positive for the presence of Leishmania-specific DNA. RFLP analysis revealed that two samples contained L. braziliensis complex DNA, one contained L. donovani complex DNA, and two samples could not be typed with the methodology used. These data suggest a potential role for the opossum as a reservoir host for zoonotic leishmaniasis in the region. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|