Vimentin Deficiency Prevents High-Fat Diet-Induced Obesity and Insulin Resistance in Mice
Autor: | Young Mi Park, Inyeong Kim, Seo Yeon Kim, Wonkyoung Cho, Goo Taeg Oh |
---|---|
Rok vydání: | 2021 |
Předmět: |
medicine.medical_specialty
Lipolysis Endocrinology Diabetes and Metabolism CD36 antigens Intermediate Filaments Blood lipids Diet High-Fat Mice chemistry.chemical_compound Insulin resistance Internal medicine Diabetes mellitus medicine Animals Vimentin Humans Glucose transporter type 4 Obesity Triglyceride biology business.industry Weight change Glucose transporter Type 2 Diabetes Mellitus medicine.disease Diet Mice Inbred C57BL Editorial Basic Research Endocrinology Diabetes Mellitus Type 2 Adipose Tissue chemistry biology.protein Original Article business GLUT4 |
Zdroj: | Diabetes & Metabolism Journal |
ISSN: | 2233-6087 2233-6079 |
Popis: | Background: Obesity and type 2 diabetes mellitus are world-wide health problems, and lack of understanding of their linking mechanism is one reason for limited treatment options. We determined if genetic deletion of vimentin, a type 3 intermediate fila ment, affects obesity and type 2 diabetes mellitus. Methods: We fed vimentin-null (Vim-/-) mice and wild-type mice a high-fat diet (HFD) for 10 weeks and measured weight change, adiposity, blood lipids, and glucose. We performed intraperitoneal glucose tolerance tests and measured CD36, a major fatty acid translocase, and glucose transporter type 4 (GLUT4) in adipocytes from both groups of mice. Results: Vim-/- mice fed an HFD showed less weight gain, less adiposity, improved glucose tolerance, and lower serum level of fasting glucose. However, serum triglyceride and non-esterified fatty acid levels were higher in Vim-/- mice than in wild-type mice. Vimentin-null adipocytes showed 41.1% less CD36 on plasma membranes, 27% less uptake of fatty acids, and 50.3% less GLUT4, suggesting defects in intracellular trafficking of these molecules. Conclusion: We concluded that vimentin deficiency prevents obesity and insulin resistance in mice fed an HFD and suggest vi mentin as a central mediator linking obesity and type 2 diabetes mellitus. |
Databáze: | OpenAIRE |
Externí odkaz: |