The INO80 complex removes H2A Z to promote presynaptic filament formation during homologous recombination
| Autor: | Boris Pfander, Stefan Jentsch, Jörg Renkawitz, Claudio A. Lademann |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA end resection Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Mutant INO80-C General Biochemistry Genetics and Molecular Biology Substrate Specificity Protein filament Histones 03 medical and health sciences chemistry.chemical_compound Replication Protein A Nucleosome DNA Breaks Double-Stranded Homologous Recombination lcsh:QH301-705.5 double-strand breaks biology H2A.Z Rad51 filament formation biology.organism_classification Molecular biology Cell biology Chromatin 030104 developmental biology Histone lcsh:Biology (General) chemistry Synapses biology.protein chromatin 570 Life sciences Rad51 Recombinase nucleosome remodeling Homologous recombination genome stability DNA |
| Zdroj: | Cell Reports, Vol 19, Iss 7, Pp 1294-1303 (2017) Cell Reports |
| Popis: | The INO80 complex (INO80-C) is an evolutionarily conserved nucleosome remodeler that acts in transcription, replication, and genome stability. It is required for resistance against genotoxic agents and is involved in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR). However, the causes of the HR defect in INO80-C mutant cells are controversial. Here, we unite previous findings using a system to study HR with high spatial resolution in budding yeast. We find that INO80-C has at least two distinct functions during HR-DNA end resection and presynaptic filament formation. Importantly, the second function is linked to the histone variant H2A.Z. In the absence of H2A.Z, presynaptic filament formation and HR are restored in INO80-C-deficient mutants, suggesting that presynaptic filament formation is the crucial INO80-C function during HR. |
| Databáze: | OpenAIRE |
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