Molecular ultrasound imaging of neutrophil membrane-derived biomimetic microbubbles for quantitative evaluation of hepatic ischemia-reperfusion injury
Autor: | Xiaoyan Miao, Xiang Li, Chaojin Chen, Zheng Zhang, Jie Ren, Yuejun Lin, Ziqing Hei, Tinghui Yin, Yujia You, Jing Yang, Weifeng Yao |
---|---|
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Orthotopic liver transplantation Neutrophils molecular ultrasound imaging Medicine (miscellaneous) Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Biomimetics medicine Animals Pharmacology Toxicology and Pharmaceutics (miscellaneous) hepatic ischemia-reperfusion injury Ultrasonography Liposome Microbubbles medicine.diagnostic_test business.industry neutrophil biomimetic medicine.disease Hepatic ischemia Rats 030104 developmental biology Liver 030220 oncology & carcinogenesis Liver biopsy Reperfusion Injury Liposomes Ultrasound imaging Neutrophil membrane business Reperfusion injury Research Paper |
Zdroj: | Theranostics |
ISSN: | 1838-7640 |
Popis: | Rationale: Early diagnosis of hepatic ischemia-reperfusion injury (HIRI), the major cause of early allograft dysfunction or primary non-function, is critical in orthotopic liver transplantation. However, liver biopsy is still the primary method for HIRI evaluation in clinical practice despite its numerous complications and shortcomings such as hemorrhage and inaccuracy. Herein, we aimed to develop a non-invasive, highly accurate, and specific method for detecting HIRI. Methods: We developed a top-down and bottom-up strategy to fabricate neutrophil biomimetic microbubbles (MBneu). Neutrophil membrane was mixed with liposomes at a defined mass ratio by sonication. The air in the vial was exchanged with perfluoropropane, and then the solution was mechanically vibrated to form MBneu. Results: MBneu retained the neutrophil proteins, preferentially targeted inflamed hepatic tissue in a rat model of HIRI, and demonstrated physicochemical properties typical of liposome-based MBs because of its artificial phospholipid content. With MBneu we can quantitively evaluate the severity of HIRI, which is helpful for early diagnosis and the prediction of outcome. In addition, MBneu was shown to be safe and showed no immunogenicity. Conclusion: We demonstrated molecular ultrasound imaging of HIRI with MBneu. This new synthesis strategy may be applied to different clinical scenarios using other cell types in the future. |
Databáze: | OpenAIRE |
Externí odkaz: |