RBP-L, a transcription factor related to RBP-Jκ
Autor: | Taku Okazaki, Yoshihito Taniguchi, Tasuku Honjo, Hisamune Kato, U Zimber-Strobl, G W Bornkamm, Shigeru Minoguchi, L J Strobl |
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Předmět: |
endocrine system
Saccharomyces cerevisiae Proteins Transcription Genetic Molecular Sequence Data Notch signaling pathway Biology DNA-binding protein Fungal Proteins Mice Transcription (biology) Animals Amino Acid Sequence Cloning Molecular Nuclear protein Molecular Biology Gene Transcription factor Regulation of gene expression Base Sequence Receptors Notch Membrane Proteins Nuclear Proteins DNA Cell Biology Molecular biology DNA-Binding Proteins Epstein-Barr Virus Nuclear Antigens Gene Expression Regulation Notch proteins Immunoglobulin J Recombination Signal Sequence-Binding Protein COS Cells Transcription Factors Research Article |
Zdroj: | Scopus-Elsevier |
Popis: | RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. Although at least four Notch genes exist in mammals, it is unknown whether each Notch requires a specific downstream signalling molecule. Here we report isolation and characterization of a mouse RBP-Jkappa-related gene named RBP-L that is expressed almost exclusively in lung, in contrast to the ubiquitous expression of RBP-Jkappa. For simplicity, we propose to call RBP-Jkappa RBP-J. The RBP-L protein bound to a DNA sequence almost identical to that of RBP-J. Surprisingly, RBP-L did not interact with any of the known four mouse Notch proteins. Although we found that RBP-L and EBNA-2 cooperated in transcriptional activation, they did not show significantly strong protein-protein interaction that can be detected by several in vivo and in vitro assays. This is again in contrast to physical association of RBP-J with EBNA-2. Several models to explain functional interaction between RBP-L and EBNA-2 are discussed. |
Databáze: | OpenAIRE |
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