Direct residue analysis of systemic insecticides and some of their relevant metabolites in wines by liquid chromatography – mass spectrometry
| Autor: | Jennifer A. Field, Vaughn M. Walton, Alix E. Robel, S. Mermer, J.D. Berset, M.L. Chien |
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| Rok vydání: | 2017 |
| Předmět: |
Insecticides
Maximum Residue Limit Metabolite Food Contamination Wine 010501 environmental sciences Tandem mass spectrometry 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry Neonicotinoids chemistry.chemical_compound Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Spiro Compounds Vitis Chromatography High Pressure Liquid 0105 earth and related environmental sciences Detection limit Aza Compounds Residue (complex analysis) Chromatography 010401 analytical chemistry Organic Chemistry Imidazoles Pesticide Residues food and beverages General Medicine Nitro Compounds 0104 chemical sciences chemistry Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 1506:45-54 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2017.05.019 |
| Popis: | A direct large volume injection (DI-LVI) high performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the quantitative determination of 16 systemic insecticides and their main plant metabolites. The assays were conducted on commercial red and white wines made from grapes grown in major wine-producing regions nationally and internationally. Using a 1:20 dilution and an injection volume of 800μL, a limit of quantitation (LOQ) of 1μgL-1 for all analytes was achieved. Matrix-matched standards (MM) were used for accurate quantitation. Imidacloprid (IMI) and methoxyfenozide (MET) were the most frequently detected parent insecticides in the wines reaching concentrations of 1-132μgL-1. Two important plant metabolites imidacloprid-olefin (IMI-OLE) and spirotetramat-enol (SPT-EN) were found at higher concentrations. In five samples SPT-EN was detected in the mgL-1 range with a maximum concentration of 16.3mgL-1 measured in a conventional white wine sample. Most "organic" wines contained no detectable or low insecticide residues, except for one sample, which showed the highest IMI (14.7μgL-1) and IMI-OLE (331μgL-1) concentrations. Considering the maximum residue limit (MRL) definition for the different insecticides, three "conventional" wine samples were non-compliant for SPT. This study highlights the importance to determine both parent and metabolite forms of systemic insecticides in the finished product. |
| Databáze: | OpenAIRE |
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