Differential Expression of α2-Adrenoceptor Subtypes in Purified Rat Pancreatic Islet A- and B-Cells
| Autor: | Noel G. Morgan, Christopher W. Perrett, Susan L.F. Chan |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
endocrine system
Cell type Transcription Genetic endocrine system diseases medicine.medical_treatment Gene Expression Stimulation Biology Polymerase Chain Reaction Glucagon Islets of Langerhans Ribonucleases Receptors Adrenergic alpha-2 medicine Animals Humans B cell geography geography.geographical_feature_category Insulin RNA Cell Biology Islet Molecular biology Rats medicine.anatomical_structure Pancreas |
| Zdroj: | Cellular Signalling. 9:71-78 |
| ISSN: | 0898-6568 |
| DOI: | 10.1016/s0898-6568(96)00096-4 |
| Popis: | In the endocrine pancreas, alpha 2-adrenoceptor stimulation reduces glucose-induced insulin secretion from islet B-cells. There is, however, controversy with regard to the effects of alpha 2-agonists on islet A-cell function. In this paper, we have used RNA samples prepared from whole rat islets and from FACS-purified rat A- and B-cells to study alpha 2-adrenoceptor subtype expression. RNase protection assays detected transcripts encoding alpha 2a and alpha 2b subtypes in the RNA pool of rat islets. Reverse transcription-PCR revealed that transcripts for all three alpha 2-adrenoceptor subtypes are present in rat islet cells in purified A-cell RNA. In contrast, RT-PCR of islet B-cell RNA yielded products corresponding to alpha 2a and alpha 2c, with no evidence for the presence of alpha 2b. Thus, the results reveal that both islet cell types express more than one receptor subtype and suggest that the distribution of the subtypes may differ between rat islet A- and B-cells. |
| Databáze: | OpenAIRE |
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