Validation of the Diagnostic and Prognostic Values of ADAMTS5 and FSTL1 in Osteoarthritis Rat Model
| Autor: | Bakheet E. M. Elsadek, Sary Kh Abd-Elghaffar, Mohamed A. Abd EL-Aziz, Amer Alkot Mostafa Elsadek, Ahmed A. Abdel-ghany, Hafez R. Madkor, Ahmed Abd Elrady Ahmed |
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| Rok vydání: | 2019 |
| Předmět: |
Oncology
Male medicine.medical_specialty Follistatin-Related Proteins Rat model Blotting Western Biomedical Engineering Physical Therapy Sports Therapy and Rehabilitation Osteoarthritis 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Immunology and Allergy Animals Humans Rats Wistar Clinical Research papers 030203 arthritis & rheumatology business.industry Public health 030229 sport sciences medicine.disease Prognosis Rats Biomarker (medicine) ADAMTS5 Protein business |
| Zdroj: | Cartilage |
| ISSN: | 1947-6043 |
| Popis: | Objective Osteoarthritis (OA) is a global public health problem and a leading cause of morbidity and disability. Due to lack of sensitive and specific tools for early OA diagnosis and predicting prognosis, the availability of new reliable and sensitive biomarkers is a widely appreciated need to identify patients at risk for incident disease or disease progression. Accordingly, our study was conducted to validate the usefulness of disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) and follistatin-like protein 1 (FSTL1) to achieve this goal. Design Fifty-four male Wistar rats were randomized into 3 groups; 24 rats were subjected to medial meniscal tear (MMT) surgery on the right knee joint (OA group), 24 rats were subjected to sham surgery (sham group), and 6 healthy rats (negative control group). Six animals from each group were sacrificed every 2 weeks. At each time point, the right knee joint of each animal was visualized radiologically, a blood sample was collected, and cartilage tissues were isolated for histopathological and western blot analysis. Results We found that the expression levels of ADAMTS5 and FSTL1 significantly increased with OA progression, especially at weeks 4, 6, and 8 after surgery. Notably, the serum levels of ADAMTS5 and FSTL1 showed significant positive correlations with each other and with the studied inflammatory markers. Conclusions Our findings suggest that ADAMTS5 and FSTL1 can serve as important and informative serological markers of disease activity in OA. However, further research is needed to validate their use for improving the diagnosis and prognosis of OA in humans. |
| Databáze: | OpenAIRE |
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