The influence of the extracellular counter-ion on the sodium-dependent, ouabain-uninhibited sodium efflux from human erythrocytes
| Autor: | R. Grant, Michael J. Dunn |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Sucrose
Erythrocytes Sodium Biophysics chemistry.chemical_element Cesium Lithium Biochemistry Ouabain Choline chemistry.chemical_compound Extracellular medicine Humans Magnesium Tromethamine chemistry.chemical_classification Radioisotopes Chromatography Cell Membrane Cell Biology Rubidium Quaternary Ammonium Compounds Kinetics Ethacrynic Acid chemistry Potassium Human erythrocytes Sodium Isotopes Efflux Counterion Sodium dependent medicine.drug |
| Zdroj: | Biochimica et biophysica acta. 352(1) |
| ISSN: | 0006-3002 |
| Popis: | 1. 1. Progressive reductions of extracellular Na + (Na O + ) reduced radioisotopic Na + efflux from human erythrocytes in the presence of ouabain. Nine different counter-ions were used to replace Na 0 + and the results were qualitatively similar. 2. 2. Ethacrynic acid always inhibited less Na + efflux in low Na 0 + solutions than in 135 mM Na 0 + solutions regardless of the counter-ion which replaced Na 0 + . 3. 3. Na + efflux was stimulated in zero Na 0 + , ouabain solutions of sucrose served as the counter-solute; ethacrynic acid eliminated this effect. 4. 4. We conclude that the reduction of ouabain-uninhibited Na + efflux as Na 0 + is reduced from 135 mM to zero is truly the result of removal of Na 0 + and not an inhibitory effect of the counter-ion used to preserve isosmolarity. Furthermore, this Na 0 + -dependent Na + efflux is practically eliminated by ethacrynic acid. |
| Databáze: | OpenAIRE |
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