Role of angiotensin II and oxidative stress on renal aquaporins expression in hypernatremic rats
| Autor: | Andrea L. Fellet, Gabriel Cao, Jorge E. Toblli, Susana Gorzalczany, Belisario E. Fernández, Marcela Pandolfo, Silvana Della Penna, Ana M. Balaszczuk, María Inés Rosón, Lorena I. Sarati, Marcelo Roberto Choi, Nicolás Martín Kouyoumdzian, Elsa Zotta |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Physiology Angiotensin Ii Blotting Western Diuresis Ciencias de la Salud Fluorescent Antibody Technique Sodium Overload Aquaporins Kidney Biochemistry Losartan Natriuresis Rats Sprague-Dawley Enos Internal medicine medicine Animals Hypernatremia biology Chemistry Aquaporin Hipernatremic Rats Angiotensin II Kidney metabolism General Medicine Tempol Apical membrane biology.organism_classification Rats Otras Ciencias de la Salud Oxidative Stress Endocrinology medicine.anatomical_structure medicine.drug |
| Zdroj: | Journal of physiology and biochemistry. 70(2) |
| ISSN: | 1877-8755 |
| Popis: | The aim of this study was to assess whether endogenous Ang II and oxidative stress produced by acute hypertonic sodium overload may regulate the expression of aquaporin-1 (AQP-1) and aquaporin-2 (AQP-2) in the kidney. Groups of anesthetized male Sprague-Dawley rats were infused with isotonic saline solution (control) or with hypertonic saline solution (Na group, 1 M NaCl), either alone or with losartan (10 mg kg(-1)) or tempol (0.5 mg min(-1) kg(-1)) during 2 h. Renal function parameters were measured. Groups of unanesthetized animals were injected intraperitoneally with hypertonic saline solution, with or without free access to water intake, Na+W, and Na-W, respectively. The expression of AQP-1, AQP-2, Ang II, eNOS, and NF-kB were evaluated in the kidney by Western blot and immunohistochemistry. AQP-2 distribution was assessed by immunofluorescence. Na group showed increased natriuresis and diuresis, and Ang II and NF-kB expression, but decreased eNOS expression. Losartan or tempol enhanced further the diuresis, and AQP-2 and eNOS expression, as well as decreased Ang II and NF-kB expression. Confocal immunofluorescence imaging revealed labeling of AQP-2 in the apical plasma membrane with less labeling in the intracellular vesicles than the apical membrane in kidney medullary collecting duct principal cells both in C and Na groups. Importantly, our data also show that losartan and tempol induces a predominantly accumulation of AQP-2 in intracellular vesicles. In unanesthetized rats, Na+W group presented increased diuresis, natriuresis, and AQP-2 expression (112 ± 25 vs 64 ± 16; *p |
| Databáze: | OpenAIRE |
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